Development of a rapid field-applicable molecular diagnostic for knockdown resistance (kdr) markers in An. gambiae

Parasites & Vectors
Vera T UnwinEmily R Adams

Abstract

The spread of insecticide resistance (IR) is a major threat to vector control programmes for mosquito-borne diseases. Early detection of IR using diagnostic markers could help inform these programmes, especially in remote locations where gathering reliable bioassay data is challenging. Most current molecular tests for genetic IR markers are only suitable for use in well-equipped laboratory settings. There is an unmet need for field-applicable diagnostics. A single-cartridge test was designed to detect key IR mutations in the major African vector of malaria, Anopheles gambiae. Developed on the portable, rapid, point-of-care compatible PCR platform - Genedrive® (genedrive® plc), the test comprises two assays which target single nucleotide polymorphisms (SNPs) in the voltage gated sodium channel (VGSC) gene that exert interactive effects on knockdown resistance (kdr): L1014F, L1014S and N1575Y. Distinct melt peaks were observed for each allele at each locus. Preliminary validation of these assays using a test panel of 70 An. gambiae samples showed complete agreement of our assays with the widely-used TaqMan assays, achieving a sensitivity and specificity of 100%. Here we show the development of an insecticide resistance detection ...Continue Reading

References

Mar 24, 2009·Transactions of the Royal Society of Tropical Medicine and Hygiene·Urvashi RamphulPie Müller
Jan 3, 2012·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Jésica FabroSheila Ons
Apr 12, 2012·Proceedings of the National Academy of Sciences of the United States of America·Christopher M JonesCraig S Wilding
Apr 8, 2014·Insect Biochemistry and Molecular Biology·Ke DongBoris S Zhorov
Feb 13, 2016·Journal of Clinical Microbiology·Shubhada ShenaiSusan E Dorman
Mar 23, 2017·Tropical Medicine & International Health : TM & IH·Sonia Ares Gomez, Albert Picado

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
genotyping
1014
1014 assay

Software Mentioned

OligoAnalyzer
Genedrive®
BLAST
GeneDrive

Related Concepts

Related Feeds

Antimalarial Agents (ASM)

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.

Antimalarial Agents

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.