Development of a rapid method for the quantitative determination of deoxynivalenol using Quenchbody

Analytica Chimica Acta
Tomoya YoshinariYoshiko Sugita-Konishi

Abstract

Quenchbody (Q-body) is a novel fluorescent biosensor based on the antigen-dependent removal of a quenching effect on a fluorophore attached to antibody domains. In order to develop a method using Q-body for the quantitative determination of deoxynivalenol (DON), a trichothecene mycotoxin produced by some Fusarium species, anti-DON Q-body was synthesized from the sequence information of a monoclonal antibody specific to DON. When the purified anti-DON Q-body was mixed with DON, a dose-dependent increase in the fluorescence intensity was observed and the detection range was between 0.0003 and 3 mg L(-1). The coefficients of variation were 7.9% at 0.003 mg L(-1), 5.0% at 0.03 mg L(-1) and 13.7% at 0.3 mg L(-1), respectively. The limit of detection was 0.006 mg L(-1) for DON in wheat. The Q-body showed an antigen-dependent fluorescence enhancement even in the presence of wheat extracts. To validate the analytical method using Q-body, a spike-and-recovery experiment was performed using four spiked wheat samples. The recoveries were in the range of 94.9-100.2%. The concentrations of DON in twenty-one naturally contaminated wheat samples were quantitated by the Q-body method, LC-MS/MS and an immunochromatographic assay kit. The LC-MS/...Continue Reading

References

Sep 3, 2004·Toxicology Letters·John Christian LarsenPeter Ruckenbauer
Mar 15, 2005·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·James J Pestka, Alexa T Smolinski
Jul 6, 2010·Analytica Chimica Acta·Tomoyuki KadotaYoshiko Sugita-Konishi
Jan 11, 2011·Interdisciplinary Toxicology·Pavlina SobrovaRene Kizek
Oct 8, 2011·Journal of the American Chemical Society·Ryoji AbeHiroshi Ueda
Jul 14, 2012·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·Koji AoyamaYoshiko Sugita-Konishi
Mar 9, 2013·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·Tomoya YoshinariYoshiko Sugita-Konishi
Nov 29, 2013·Journal of AOAC International·Andre L AlbertAlan H Davis

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Citations

Oct 18, 2018·Drug Testing and Analysis·Ako SasaoYoko Nishitani
Jun 18, 2020·Methods and Protocols·Hee-Jin JeongHiroshi Ueda
Feb 13, 2021·Sensors·Jinhua Dong, Hiroshi Ueda

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