PMID: 6983543Nov 12, 1982Paper

Development of a rapid microELISA assay for screening hybridoma supernatants for murine monoclonal antibodies

Journal of Immunological Methods
H H HandleyI Royston

Abstract

A micro enzyme-linked immunosorbent assay (ELISA) utilizing a filtration method has been developed which allows the rapid, simple, and sensitive detection of monoclonal antibodies that recognize either soluble or cell surface antigens. This assay involves the immobilization of target cells (or soluble antigen) onto glass fiber filter discs followed by an incubation with the test hybridoma supernatant and subsequent analysis by ELISA. A specially designed 96-well filtration device is employed which serves both as an incubation chamber and as a filtration manifold. This microELISA requires small volumes of antiserum, few target cells, and can be completed in less than 2 h. This assay is well suited for the rapid screening of murine hybridoma supernatants and can be adapted to detect monoclonal antibodies from other species.

Citations

Sep 16, 1983·Journal of Immunological Methods·S L KunkelP A Ward
Mar 11, 1983·Journal of Immunological Methods·M C GlassyI Royston
Oct 12, 1984·Journal of Immunological Methods·M StyaW H Beierwaltes
Jul 16, 1985·Journal of Immunological Methods·M C Glassy, C D Surh
Jul 11, 1986·Journal of Immunological Methods·P A HessianD G Palmer
Mar 1, 1990·Veterinary Immunology and Immunopathology·D R KrawiecS J Dugan
Jan 1, 1989·Journal of Pharmaceutical and Biomedical Analysis·J M Apathy, R R Bowsher
Dec 1, 1995·Insect Biochemistry and Molecular Biology·A OhnishiS Sekiguchi
Oct 1, 1986·Clinical Immunology and Immunopathology·D L ShawlerR O Dillman
Jan 1, 1987·Critical Reviews in Clinical Laboratory Sciences·P J MoldofskyN D Hammond
Mar 1, 1988·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·C D SurhM E Gershwin
Jun 15, 1985·International Journal of Cancer. Journal International Du Cancer·D M ThomsonG Shenouda

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