Development of a real-time microchip PCR system for portable plant disease diagnosis

PloS One
Chiwan KooArum Han

Abstract

Rapid and accurate detection of plant pathogens in the field is crucial to prevent the proliferation of infected crops. Polymerase chain reaction (PCR) process is the most reliable and accepted method for plant pathogen diagnosis, however current conventional PCR machines are not portable and require additional post-processing steps to detect the amplified DNA (amplicon) of pathogens. Real-time PCR can directly quantify the amplicon during the DNA amplification without the need for post processing, thus more suitable for field operations, however still takes time and require large instruments that are costly and not portable. Microchip PCR systems have emerged in the past decade to miniaturize conventional PCR systems and to reduce operation time and cost. Real-time microchip PCR systems have also emerged, but unfortunately all reported portable real-time microchip PCR systems require various auxiliary instruments. Here we present a stand-alone real-time microchip PCR system composed of a PCR reaction chamber microchip with integrated thin-film heater, a compact fluorescence detector to detect amplified DNA, a microcontroller to control the entire thermocycling operation with data acquisition capability, and a battery. The enti...Continue Reading

References

Aug 1, 1995·Applied and Environmental Microbiology·C P WoloshukG A Payne
Jun 5, 1999·Microbiology and Molecular Biology Reviews : MMBR·C L BenderD C Gross
Jan 5, 2000·Applied and Environmental Microbiology·R J St LegerB Shams-Pirzadeh
Jan 24, 2004·Journal of Virological Methods·N BoonhamR A Mumford
Jan 28, 2005·Molecular Plant-microbe Interactions : MPMI·M RepB J C Cornelissen
Jul 27, 2005·Proceedings of the National Academy of Sciences of the United States of America·Helene FeilSteven E Lindow
May 17, 2006·Trends in Ecology & Evolution·Pamela K AndersonPeter Daszak
Feb 12, 2008·Current Protocols in Molecular Biology·K Wilson
May 10, 2008·Biomedical Microdevices·Torsten Lund-OlesenMikkel Fougt Hansen
Mar 31, 2009·Analytica Chimica Acta·Yonghao Zhang, Pinar Ozdemir
May 3, 2011·Biosensors & Bioelectronics·Sandra JulichSabine Werres
May 25, 2011·Lab on a Chip·Xu ShiDeirdre R Meldrum
May 28, 2011·Biomedical Microdevices·Xianbo QiuHaim H Bau
Jul 12, 2011·Biotechnology Advances·Seungkyung ParkSamuel Yang
Jan 10, 2012·Biosensors & Bioelectronics·Dolores VerdoyGarbiñe Olabarría
Feb 15, 2013·Lab on a Chip·Chen-Min ChangGwo-Bin Lee

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Citations

Jan 30, 2018·Biomedical Microdevices·Sangdo JeongJong-Hyun Lee
May 6, 2014·Applied and Environmental Microbiology·Weihong XuRichard W Hyman
Nov 7, 2018·Biomedical Engineering Online·Jong-Dae KimChan-Young Park
Nov 7, 2018·Biomedical Engineering Online·Deuk-Ju LeeChan-Young Park
Feb 20, 2020·Current Protocols in Plant Biology·Rajesh PaulQingshan Wei
Nov 27, 2018·Royal Society Open Science·Young Mi KwonElizabeth P Murchison
Jan 4, 2018·Journal of Applied Microbiology·M M NikitinA G Golikov
Nov 17, 2020·Frontiers in Plant Science·Paolo Baldi, Nicola La Porta
Mar 16, 2021·Emerging Topics in Life Sciences·Gonçalo SilvaNeil Boonham
Feb 20, 2019··Ji Sung ParkHye Jeong Song

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Methods Mentioned

BETA
ELISA
PCR
electrophoresis
chips
chip

Software Mentioned

COMSOL Multiphysics® simulation
MPLAB IDE
COMSOL Multiphysics®

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