Development of a Real Time PCR system for detection of Penicillium nordicum and for monitoring ochratoxin A production in foods by targeting the ochratoxin polyketide synthase gene

Systematic and Applied Microbiology
Rolf GeisenPaul Färber

Abstract

A 750 bp DNA fragment from the genome of Penicillium nordicum was isolated using degenerated primers for polyketide synthase genes. All analyzed P. nordicum strains possessed the fragment, whereas the closely related ochratoxinogenic P. verrucosum strains did not. The nucleotide sequence of the fragment has been determined. It shows homology to the pksL2 polyketide synthase gene from A. parasiticus. The deduced amino acid sequence of this fragment shows high homology to several other fungal polyketide synthases. An expression analysis of this gene by Reverse Transcription Real Time PCR demonstrates that this putative polyketide synthase gene (otapksPN) is highly induced under ochratoxin A producing conditions, but only to low levels under non-producing conditions. A Real Time PCR system based on the otapksPN sequence has been used to monitor growth and ochratoxin A production of P. nordicum in wheat. A strong correlation between the copy numbers of the otapksPN gene and the colony forming units (cfu) has been observed. In addition there was a strong congruence between otapksPN gene expression and ochratoxin A production in wheat. According to these results the fragment is obviously part of a polyketide synthase (otapksPN) which...Continue Reading

Citations

Jul 16, 2005·Food Additives and Contaminants·L NiessenR Geisen
Aug 15, 2009·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·M V SelmaR Aznar
Feb 22, 2011·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·E BerniE Spotti
Apr 1, 2010·Toxins·André el Khoury, Ali Atoui
Dec 4, 2010·Genetics and Molecular Research : GMR·M A MoslemM A Elnagaer
Jan 22, 2013·International Journal of Food Microbiology·Abdelhamid AbbasAlan D W Dobson
Jan 14, 2012·International Journal of Food Microbiology·Ali AtouiAhmed Lebrihi
Jun 16, 2009·International Journal of Food Microbiology·Markus Schmidt-HeydtRolf Geisen
Sep 3, 2014·Journal of Microbiological Methods·Thai-Hoang LeZhi Zhou
Nov 19, 2005·Chemico-biological Interactions·Diana RingotYvan Larondelle
Jun 25, 2005·International Journal of Food Microbiology·Huy Phong DaoAhmed Lebrihi
Apr 10, 2016·International Journal of Food Microbiology·Massimo FerraraAntonia Susca
Apr 5, 2014·International Journal of Food Microbiology·Antonia GalloGiancarlo Perrone
Mar 1, 2005·Mycotoxin Research·A KarolewiezR Geisen
Mar 29, 2007·Natural Product Reports·Dirk Hoffmeister, Nancy P Keller
Mar 15, 2015·Scientific Reports·F Javier CabañesArmand Sánchez
Mar 1, 2007·Mycotoxin Research·M Schmidt-Heydt, R Geisen
Jan 6, 2017·Chinese Medicine·Run-Sheng ZhengWei-Wen Chen
Mar 3, 2021·International Journal of Food Microbiology·Antonia SuscaRobert H Proctor

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