Development of a real-time quantitative PCR assay using a TaqMan minor groove binder probe for the detection of α-lactalbumin in food

Journal of Dairy Science
Guan XiaoChen Qin

Abstract

Here, we report the development of a real-time PCR assay using a TaqMan minor groove binder (MGB, Genecore, NCBI: AF249896.1, 806-820) probe and primer sets designed to recognize the α-lactalbumin gene from the cow (Bos taurus). We evaluated the efficacy of this assay for detecting and quantifying cow α-lactalbumin in commercial foods. Our results demonstrated that the developed method was highly sensitive and showed high specificity for cow milk, with consistent detection of 0.05 ng of bovine DNA. We tested 42 commercial food samples with or without cow milk listed as an ingredient by using the developed assay. Among the 42 samples, 26 products that listed milk as an ingredient and 3 products might contain milk showed positive signals, whereas the other 9 products that did not contain milk and 4 products that might contain milk tested negative. Therefore, this method could be widely used for the detection of cow milk allergens in food.

References

Jan 1, 1995·Pediatric Pulmonology. Supplement·L BusincoB Bellioni
Aug 1, 1993·European Journal of Pediatrics·J J SchranderA D Kester
Oct 8, 2005·The Journal of Allergy and Clinical Immunology·Kristiina M SaarinenErkki Savilahti
May 29, 2009·Analytical and Bioanalytical Chemistry·Elena ScaravelliArjon J van Hengel
Feb 24, 2010·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Montserrat EspineiraFrancisco J Santaclara
Jun 23, 2010·Molecular Nutrition & Food Research·Phil E JohnsonAlan R Mackie
Dec 28, 2010·Journal of the American Dietetic Association·Joshua A BoyceMatthew J Fenton
Feb 22, 2012·Journal of Agricultural and Food Chemistry·Beatriz HerreroMontserrat Espiñeira
Feb 16, 2013·Food Chemistry·Elisa IniestoRosario Linacero
Jun 21, 2015·Analytica Chimica Acta·V Ruiz-Valdepeñas MontielJ M Pingarrón

❮ Previous
Next ❯

Citations

Sep 1, 2020·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Thomas HolzhauserSimon D Flanagan
Jan 1, 2018·Comprehensive Reviews in Food Science and Food Safety·Caterina VillaIsabel Mafra
Dec 19, 2020·Comprehensive Reviews in Food Science and Food Safety·Linglin FuYanbo Wang
Jul 22, 2021·Comprehensive Reviews in Food Science and Food Safety·Mati Ullah KhanZhenxing Li

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.