Development of a reliable low-cost controlled cooling rate instrument for the cryopreservation of hematopoietic stem cells.

Cytotherapy
Zhiquan ShuDayong Gao

Abstract

An optimal cooling rate is one of the critical factors influencing the survival of cells during cryopreservation. We describe a novel device, called the box-in-box, that has been developed for optimal cryopreservation of human hematopoietic stem cells (HSC). This work presents the design of the device, a mathematical formulation describing the expected temperature histories of samples during the freezing process, along with actual experimental results of thermal profile tests. In experiments, when the box-in-box device was transferred from room temperature to a -80 degrees C freezer, a cooling rate of -1 to -3.5 degrees C/min, which has been widely used for the cryopreservation of HSC, was achieved. In order to evaluate this device further, HSC cryopreservation was compared between the box-in-box device and a commercially available controlled-rate freezer (CryoMed). The experimental data, including total cell population and CD34(+) hematopoietic progenitor cell recovery rates, viability and cell culture colony assays, showed that the box-in-box worked as well as the CryoMed instrument. There was no significant difference in either survival rate or the culture/colony outcome between the two devices. The box-in-box device can wor...Continue Reading

References

Mar 30, 1995·International Journal of Radiation Oncology, Biology, Physics·P MauchH J Deeg
Jan 1, 1995·Annual Review of Cell and Developmental Biology·S J MorrisonI L Weissman
Dec 2, 2005·Obstetrics and Gynecology·Kenneth J Moise

❮ Previous
Next ❯

Citations

Aug 31, 2012·Journal of Clinical Microbiology·Zhiquan ShuDayong Gao
Jan 24, 2013·Expert Opinion on Biological Therapy·Sreedhar ThirumalaErik J Woods
Mar 24, 2015·Bioscience Reports·Imran UllahGyu Jin Rho
Jan 18, 2011·Cytotherapy·Robert C Caskey, Kenneth W Liechty
Nov 11, 2019·Annals of Biomedical Engineering·David A Castilla-CasadiegoJorge Almodovar
Mar 1, 2021·Transfusion Medicine Reviews·Suria JahanNicolas Pineault

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.