Development of a removable head fixation device for longitudinal behavioral and imaging studies in rats

Journal of Neuroscience Methods
Yuki HoriTakashi Hanakawa

Abstract

In some behavioral neuroscience studies, an attachment is surgically fixed onto the head of an awake animal to allow the animal to perform learning tasks repeatedly in the same position in a task-training system. A recently developed task-training system enables operant conditioning of head-fixed rats within only a few days, and this system has been rigorously applied to record learning-associated neural activity using electrophysiological techniques. However, the head attachment of this device is made of metal and thus is not suitable for simultaneous brain imaging studies with X-ray computed tomography (CT), magnetic resonance imaging (MRI) or positron emission tomography (PET). We developed a novel head fixation device with a removable attachment to position the rat head precisely in both imaging and training devices across different sessions. The device consisted of a removable attachment, a clamp and a stage, all of which were made of PET/MRI compatible acrylic resin. We tested the usefulness of the device with (18)F-fluorodeoxyglucose (FDG) PET and CT. The new device did not substantially affect (18)F-FDG PET images. Repositioning of the rat's head across sessions and experimenters was at a level of submillimeter accuracy...Continue Reading

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