Development of a reverse transcription quantitative polymerase chain reaction-based assay for broad coverage detection of African and Asian Zika virus lineages.

Virologica Sinica
Yang YangYuhai Bi

Abstract

The Zika virus (ZIKV) is an arbovirus that has spread rapidly worldwide within recent times. There is accumulating evidence that associates ZIKV infections with Guillain-Barré Syndrome (GBS) and microcephaly in humans. The ZIKV is genetically diverse and can be separated into Asian and African lineages. A rapid, sensitive, and specific assay is needed for the detection of ZIKV across various pandemic regions. So far, the available primers and probes do not cover the genetic diversity and geographic distribution of all ZIKV strains. To this end, we have developed a one-step quantitative reverse transcription polymerase chain reaction (qRT-PCR) assay based on conserved sequences in the ZIKV envelope (E) gene. The detection limit of the assay was determined to be five RNA transcript copies and 2.94 × 10-3 50% tissue culture infectious doses (TCID50) of live ZIKV per reaction. The assay was highly specific and able to detect five different ZIKV strains covering the Asian and African lineages without nonspecific amplification, when tested against other flaviviruses. The assay was also successful in testing for ZIKV in clinical samples. Our assay represents an improvement over the current methods available for the detection ZIKV and ...Continue Reading

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Citations

Feb 7, 2018·Frontiers in Microbiology·Raj K SinghRubén Bueno-Marí
Feb 10, 2021·Medicinal Research Reviews·Xianlong ZhangTony D James
Aug 14, 2021·Nature Metabolism·Huanhuan PangZeping Hu

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Datasets Mentioned

BETA
AY632535

Methods Mentioned

BETA
PCR
ELISAs

Software Mentioned

Primer Premier
Clustal X
[UNK] Real - Time PCR

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