Development of a robust LC-MS/MS method for determination of desmosine and isodesmosine in human urine

Journal of Oleo Science
Kohei ShiraishiKazuhiko Iba

Abstract

Desmosine (DES) and isodesmosine (IDES) are both pyridinium amino acid isomers that serve as cross-linking molecules binding the polymeric chains of amino acids into elastin. Found in urine, they are markers for the degradation of elastin which occurs in chronic obstructive pulmonary disease (COPD). In this study, a robust method using ultra-performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS) with selected reaction monitoring (SRM) mode was developed for the analysis of DES and IDES in human urine. Pyridylethyl-cysteine (PE-Cys) as internal standard (I.S.) was employed for the quantification of DES and IDES. The analytes and I.S. were extracted by solid-phase extraction with Oasis MCX cartridges and separated on an AccQ-Tag Ultra column. The assay was accurate (-6.8% to 14.5%) and precise (2.8% to 13.8%) within the concentration range of 1 to 250 pmol/mL. Moreover, the recovery and stability (working/ I.S. solution, urine samples with added elastin, and pretreated sample) was investigated, and these parameters were found acceptable. The UPLC-MS/MS method was validated and had good reproducibility and stability for the quantification of DES and IDES, which requires only 100 mL of human urine. This ...Continue Reading

References

Oct 1, 1980·The Journal of Clinical Investigation·R M SeniorR P Mecham
Jul 8, 2000·The European Respiratory Journal·S ViglioM Luisetti
Apr 12, 2002·The International Journal of Biochemistry & Cell Biology·Franca CocciGiuseppe Lungarella
Oct 18, 2003·Proceedings of the National Academy of Sciences of the United States of America·Shuren MaYong Y Lin
Apr 7, 2005·Biopolymers·Gautier MoroyStéphanie Héry-Huynh
Jun 1, 2005·Journal of Pharmaceutical and Biomedical Analysis·Stephen A C Wren
Jul 9, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Mona I ChurchwellDaniel R Doerge
Jul 7, 2007·Physiological Reviews·Toshinori Yoshida, Rubin M Tuder
Jan 31, 2009·Analytical Chemistry·Michel BoutinPierre Thibault

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Citations

Sep 3, 2011·Analytical and Bioanalytical Chemistry·Joanna Perła-KajánHieronim Jakubowski
Nov 18, 2011·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Neil A DevenportColin S Creaser
Apr 22, 2015·Bioorganic & Medicinal Chemistry Letters·Takahiro TanigawaToyonobu Usuki
Aug 1, 2018·Analytical and Bioanalytical Chemistry·Pratikkumar RathodEmmanuel J Chang
Feb 7, 2020·Chirality·Daria A BautToyonobu Usuki
Feb 23, 2011·Bioanalysis·Joanne MatherRobert S Plumb
Feb 23, 2012·Chemical Communications : Chem Comm·Toyonobu UsukiYong Y Lin

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