Development of a ruthenium(II) complex-based luminescent probe for hypochlorous acid in living cells

Inorganic Chemistry
Run ZhangJingli Yuan

Abstract

A novel Ru(II) complex, [Ru(bpy)2(DNPS-bpy)](PF6)2 (bpy: 2,2'-bipyridine, DNPS-bpy: 4-(2,4-dinitrophenylthio)methylene-4'-methyl-2,2'-bipyridine), has been designed and synthesized as a highly sensitive and selective luminescence probe for the recognition and detection of hypochlorous acid (HOCl) in living cells by exploiting a "signaling moiety-recognition linker-quencher" sandwich approach. The complex possesses large stokes shift (170 nm), long emission wavelength (626 nm), and low cytotoxicity. Owing to the effective photoinduced electron transfer (PET) from Ru(II) center to the electron acceptor, 2,4-dinitrophenyl (DNP), the red-emission of bipyridine-Ru(II) complex was completely withheld. In aqueous media, HOCl can trigger an oxidation reaction to cleave the DNP moiety from the Ru(II) complex, which results in the formation of a highly luminescent bipyridine-Ru(II) complex derivative, [Ru(bpy)2(COOH-bpy)](PF6)2 (COOH-bpy: 4'-methyl-2,2'-bipyridyl-4-carboxylic acid), accompanied by a 190-fold luminescence enhancement. Cell imaging experimental results demonstrated that [Ru(bpy)2(DNPS-bpy)](PF6)2 is membrane permeable, and can be applied for capturing and visualizing the exogenous/endogenous HOCl molecules in living cell s...Continue Reading

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Citations

Aug 17, 2014·Biochimica Et Biophysica Acta·Adam MechlerConor F Hogan
Nov 7, 2016·Journal of Photochemistry and Photobiology. B, Biology·Dan WanYun-Jun Liu
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Apr 9, 2015·Dalton Transactions : an International Journal of Inorganic Chemistry·Zhiqiang YeJingli Yuan
Jun 25, 2014·Dalton Transactions : an International Journal of Inorganic Chemistry·Mei-Jin LiGuonan Chen
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Apr 18, 2014·Dalton Transactions : an International Journal of Inorganic Chemistry·Zhiqiang YeJingli Yuan
Nov 20, 2016·Chemistry, an Asian Journal·Sandip V MulayDavid G Churchill
Aug 27, 2014·Chemical Communications : Chem Comm·Shahi Imam RejaManoj Kumar
Jul 2, 2020·The Analyst·Shaoqing DongChao Lu
Apr 21, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xiangli LiuJingli Yuan

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