Development of a selective culture medium for primary isolation of the main Brucella species

Journal of Clinical Microbiology
María Jesús de MiguelJosé M Blasco

Abstract

Bacteriological diagnosis of brucellosis is performed by culturing animal samples directly on both Farrell medium (FM) and modified Thayer-Martin medium (mTM). However, despite inhibiting most contaminating microorganisms, FM also inhibits the growth of Brucella ovis and some B. melitensis and B. abortus strains. In contrast, mTM is adequate for growth of all Brucella species but only partially inhibitory for contaminants. Moreover, the performance of both culture media for isolating B. suis has never been established properly. We first determined the performance of both media for B. suis isolation, proving that FM significantly inhibits B. suis growth. We also determined the susceptibility of B. suis to the antibiotics contained in both selective media, proving that nalidixic acid and bacitracin are highly inhibitory, thus explaining the reduced performance of FM for B. suis isolation. Based on these results, a new selective medium (CITA) containing vancomycin, colistin, nystatin, nitrofurantoin, and amphotericin B was tested for isolation of the main Brucella species, including B. suis. CITA's performance was evaluated using reference contaminant strains but also field samples taken from brucella-infected animals or animals s...Continue Reading

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Citations

Sep 17, 2014·BMC Veterinary Research·Marcos Isidoro-AyzaMariano Domingo
Mar 27, 2012·Veterinary Immunology and Immunopathology·Pilar M MuñozRaúl C Mainar-Jaime
Nov 22, 2014·Journal of Veterinary Pharmacology and Therapeutics·Lucía Dieste-PérezPilar M Muñoz
Oct 5, 2011·Research in Veterinary Science·Ana Cristina FerreiraMaria Inácia Corrêa de Sá
Feb 11, 2015·Journal of Microbiological Methods·Lucía Dieste-PérezPilar M Muñoz
Mar 21, 2015·BioMed Research International·Denis Rwabiita MugiziUlf Magnusson
May 22, 2016·International Journal of Immunopathology and Pharmacology·Alaa Bassuny IsmaelAbdel-Rahman A Alhumiany
Nov 21, 2017·Journal of Neuropathology and Experimental Neurology·Frank O BastianPhilip H Elzer
Nov 15, 2019·Clinical Microbiology Reviews·Pablo YagupskyJuan D Colmenero
Aug 10, 2017·PloS One·Gabriela Hernández-MoraEdgardo Moreno
Mar 5, 2020·The Onderstepoort Journal of Veterinary Research·Maphuti B LedwabaHenriette Van Heerden
Jun 15, 2017·Human Cell·Tamara D KolokoltsovaGennady T Sukhikh
Feb 2, 2021·Frontiers in Microbiology·Leticia Lázaro-AntónAmaia Zúñiga-Ripa

Related Concepts

Microbicides
Bacteriological Techniques
Brucella
Brucellosis, Pulmonary
Selection, Genetic
Antibiotics
Brucella abortus bacterium
Brucellosis
Colistin
Disease Susceptibility

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