Development of a selective media for detecting Campylobacter spp. in chicken carcasses using avibactam supplemented mCCDA.

Food Science and Biotechnology
Binn Kim, Kun-Ho Seo

Abstract

Campylobacter spp. are major causes of gastrointestinal infections worldwide, and are commonly identified using modified-charcoal-cefoperazone-deoxycholate agar (mCCDA). However, the efficacy of this screening technique is often hindered by overgrowth of competing flora, such as extended-spectrum β-lactamase (ESBL)-producing Escherichia coli. Thus, in the present study we supplemented mCCDA with a recently developed ESBL inhibitor, avibactam (A-mCCDA). We inoculated mCCDA and A-mCCDA plates with 25 strains each of Campylobacter spp. and ESBL-producing E. coli, and thereby determined that the optimum avibactam concentration required to inhibit ESBL-producing E. coli was 0.0625 mg/L. At this concentration, a significantly higher proportion of Campylobacter spp. was isolated using A-mCCDA compared to that using mCCDA (P < 0.05). Thus, the results of the present study support the use of A-mCCDA to improve current Campylobacter screening methods.

References

Jul 4, 2012·Proceedings of the National Academy of Sciences of the United States of America·David E EhmannStewart L Fisher
May 30, 2015·International Journal of Antimicrobial Agents·Marta Fernández-MartínezUNKNOWN Spanish Network for the Research in Infectious Diseases (REIPI)
Feb 26, 2016·International Journal of Food Microbiology·Jung-Whan ChonKun-Ho Seo
Jul 13, 2016·Antimicrobial Agents and Chemotherapy·Martine I AbboudJean-Marie Frère
Oct 12, 2017·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Aimee L GeisslerBarbara E Mahon
Feb 27, 2018·EFSA Journal·UNKNOWN European Food Safety Authority, UNKNOWN European Centre for Disease Prevention and Control

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