Development of a sensitive enzyme immunoassay for human epidermal growth factor (urogastrone)

Clinica Chimica Acta; International Journal of Clinical Chemistry
M KurobeK Hayashi

Abstract

A sensitive two-site enzyme immunoassay (EIA) for human epidermal growth factor (hEGF) was developed, based on the sandwiching of an antigen between anti-hEGF IgG-coated polystyrene beads and anti-hEGF Fab'-linked peroxidase complex (horseradish peroxidase, EC. 1.11.1.7). This method has four advantages: the anti-hEGF Fab'-linked peroxidase complex is more stable than 125I-labelled antibody; the procedure is simple and rapid compared to bioassay; its discriminatory sensitivity is as low as 0.1 pg/assay tube; and serial dilution curves of unextracted human serum and urine samples all paralleled that of standard hEGF. The validity of the measurement of hEGF-like immunoreactivity in human serum and plasma is discussed.

References

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Citations

Jan 1, 1987·Virchows Archiv. A, Pathological Anatomy and Histopathology·M MoriK Kato
Mar 1, 1991·Applied Biochemistry and Biotechnology·A SekiI Karube
Sep 15, 1987·Clinica Chimica Acta; International Journal of Clinical Chemistry·Y AbeS Kimura
Dec 15, 1988·Clinica Chimica Acta; International Journal of Clinical Chemistry·M KurobeK Hayashi
Oct 1, 1988·Regulatory Peptides·A L MattilaJ Perheentupa
Apr 14, 1986·Biochemical and Biophysical Research Communications·K MoriK Hayashi
Apr 1, 1991·Clinical Biochemistry·B OrsiniC Surrenti

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