PMID: 3320573Dec 1, 1987Paper

Development of a sensitive enzymeimmunoassay (EIA) for progesterone determination in unextracted bovine plasma using the second antibody technique

Journal of Steroid Biochemistry
B S PrakashD F van de Wiel

Abstract

A simple direct enzymeimmunoassay (EIA) on microtiter plates for plasma progesterone using the second antibody coating technique and horseradish peroxidase (HRP) as the enzyme label (EIA-HRP) is described and compared with an identical EIA procedure which employed alkaline phosphatase (AP) as the enzyme label (EIA-AP). The assays used antiserum raised against progesterone-7-carboxyethlthioether-BSA in rabbits. Both systems were further compared with the conventional direct progesterone radioimmunoassay (RIA) in regular use. The enzymes HRP and AP were coupled to progesterone-6 beta-hydroxy-hemisuccinate by a mixed anhydride method. While the precision of EIA-HRP was comparable to RIA, the sensitivity in terms of the lowest detection limit obtained in EIA-HRP was about 10 times better than that seen in RIA. Progesterone estimates from plasma samples in EIA-HRP showed good correlation (r = 0.94) with the RIA values and the levels measured in the two systems were identical. Progesterone estimates from plasma samples in EIA-AP were at least three times higher than those obtained by either EIA-HRP or RIA. Thus, only the EIA-HRP but not the EIA-AP was suitable for the reliable direct measurement of progesterone in plasma.

References

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Citations

Aug 15, 1989·Clinica Chimica Acta; International Journal of Clinical Chemistry·K M RajkowskiN Cittanova
Mar 1, 1991·The Journal of Steroid Biochemistry and Molecular Biology·J el Jabri
Nov 24, 2005·Prostaglandins & Other Lipid Mediators·E LiszewskaJ Kotwica
Jul 7, 2000·Theriogenology·H BollweinR Stolla
Jun 18, 2002·Theriogenology·H BollweiR Stolla
Jun 18, 2002·Theriogenology·H BollweinR Stolla
Feb 1, 1997·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·G A BubenikL Bartos
Apr 12, 2002·Animal Reproduction Science·Gaby HirsbrunnerA Steiner
Jul 11, 2002·Animal Reproduction Science·Mehmet Akif CamErdogan Selcuk
Apr 25, 2003·The Journal of Steroid Biochemistry and Molecular Biology·M SchoenfelderR Einspanier
Nov 1, 1992·The Journal of Dairy Research·R M BruckmaierJ W Blum
Sep 24, 2004·Journal of Animal Physiology and Animal Nutrition·F J SchweigertJ Gropp
May 22, 2002·Journal of Veterinary Medicine. A, Physiology, Pathology, Clinical Medicine·S YildizM Uzun
Mar 12, 2002·Reproduction in Domestic Animals = Zuchthygiene·T LengwinatW Jöchle
Apr 27, 2002·Reproduction in Domestic Animals = Zuchthygiene·S HalilogluZ Bulut
Nov 16, 2012·BMC Veterinary Research·Beatrice Wenzinger, Ulrich Bleul
Oct 1, 2010·The Journal of Reproduction and Development·Marion PiechottaHeinrich Bollwein
Sep 13, 2008·The Journal of Reproduction and Development·Alexandra RauchHeinrich Bollwein

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