PMID: 22577660May 12, 2012Paper

Development of a sensitive method for simultaneous determination of fluticasone propionate and salmeterol in plasma samples by liquid chromatography-tandem mass spectrometry

Biomedical Chromatography : BMC
Luigi SilvestroIsabela Tarcomnicu

Abstract

A new method for the fast simultaneous quantification of fluticasone propionate and salmeterol from plasma samples by liquid chromatography-tandem mass spectrometry, with adequate sensitivity for pharmacokinetic applications, was developed and validated. The chromatographic separation and mass-spectrometric parameters were optimized for the retention and detection of the two compounds, despite quite different structures and properties. Two columns connected in series were used, cation-exchange (Zorbax 300-SCX, 5 cm x 2.1 mm, 5 μm) and octadecyl (Discovery HSC₁₈, 10 cm x 2.1 mm, 5 μm). The mass-spectrometric interface was operated in negative electrospray ionization mode; high sensitivity and lesser matrix effects were obtained, permitting smaller consumption of plasma. The sample preparation was based on supported liquid-liquid extraction in 96-well format plates that provided clean samples with a simplified procedure that was suitable for automation. The method was validated according to regulatory guidelines, by assessing lower limits of quantification, selectivity, linearity, accuracy, precision, extraction recoveries and matrix effects. A comparison with two other methods for the separate determination of fluticasone propio...Continue Reading

References

Feb 15, 2000·American Journal of Respiratory and Critical Care Medicine·G ShapiroT Shah
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Mar 22, 2000·Journal of Pharmaceutical and Biomedical Analysis·S KrishnaswamiG Hochhaus
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Jul 13, 2007·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Vladimír Cápka, Spencer J Carter
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Jan 5, 2011·Biomedical Chromatography : BMC·P GarciaY Bonnaire

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Citations

Aug 25, 2015·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·K SouleleV Karalis
Apr 5, 2017·Biopharmaceutics & Drug Disposition·Konstantina SouleleVangelis Karalis
Feb 28, 2016·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Hanan A MereyBadr A El-Zeany

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