Development of a sensitive polyclonal antibody-based competitive indirect ELISA for determination of citrinin in grain-based foods

Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
Gurmit SinghTerry Koerner

Abstract

A sensitive competitive indirect enzyme-linked immunosorbent assay (ciELISA) was developed for the detection and quantification of citrinin (CIT) in grain-based food samples. The limit of quantification (IC20) of the established method was 0.10 ± 0.02 ng mL-1, with the limit of detection (IC10) being 0.04 ± 0.007 ng mL-1 in wheat and corn flour matrices with a coefficient of variation (CV) less than 20%. The assay was very specific to CIT and showed no cross-reactivity with other mycotoxins (OTA, T-2 toxin, HT-2 toxin, DON, patulin and zearalenone). In spiked wheat and corn flours, the recoveries ranged from 86.6% to 115.6% with CVs of less than 20%. The effectiveness of this method was verified by participating in a proficiency test (PT) from the Food Analysis Performance Assessment Scheme (FAPAS) 17181 corn flour. A successful z-score (-0.6) for this PT sample showed that the present method is comparable to the instrumental methods used by other laboratories in the PT testing scheme. A small survey of grain-based foods was conducted using this method and CIT was detected in 43% of the samples up to a concentration of 17.7 ng g-1. This method is suitable for sensitive and rapid quantitation of citrinin in wheat and corn matrices.

References

Mar 29, 2002·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Pin-Yen Shu, Cheng-Huang Lin
Oct 2, 2007·Journal of Immunological Methods·Youxiang ZhouFusheng Chen
Nov 22, 2008·Journal of Immunological Methods·Yi FengZhixian Gao
Jul 1, 2015·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·José F Huertas-PérezLaura Gámiz-Gracia
Jul 28, 2015·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Ádám TölgyesiTheresa Zwickel
Oct 20, 2017·Food Additives & Contaminants. Part B, Surveillance·Jelka PleadinKsenija Markov

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Citations

Sep 7, 2021·Analytical Methods : Advancing Methods and Applications·Nicolás Mateo González-LópezZuly Jenny Rivera-Monroy

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Methods Mentioned

BETA
enzyme-linked immunosorbent assays
ELISA
chips

Software Mentioned

Ascent
GraphPad
GraphPad prism

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