Development of a serum-free and heat-sterilizable medium and continuous high-density cell culture.

Cytotechnology
Y MinamotoK Mitsugi

Abstract

We tried to establish a new serum-free and heat-sterilizable medium, based on our serum-free medium in which many lymphoblastoid cells and hybridoma could grow as well as in a conventional serum-containing medium.As is well-known, L-glutamine (L-Gln) is one of the most heat-labile but essential components for cell growth. As a substitute for L-Gln, dipeptide such as Gly-L-Gln or L-Ala-L-Gln, which was quite stable even after autoclaving, was found to be utilizable for mammalian cell growth. The L-Gln dipeptide-containing serum-free medium was quite stable in a solution even after storing at 37°C for 4 months. In the serum-free medium containing L-Ala-L-Gln, mouse hybridola could grow and produce more antibody than in RPMI 1640+10% FBS.It has been proved that BSA and transferrin, which are also heat-labile but essential for the growth of various cell lines, can be substituted by heat-stable alpha-cyclodextrin and cholesterol, and Fe-gluconate, respectively. Insulin has also proved to be heat stable in a solution of Fe-gluconate. We thus established a new serum-free medium, all the components of which could be heat-sterilizable.Moreover, by adding EGF and BSA but without the adhesion factor included in FBS, the serum-free medium ...Continue Reading

References

Jan 1, 1968·Proceedings of the Society for Experimental Biology and Medicine·I YamaneK Jimbo
Feb 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·H MurakamiT Kano-Sueoka
Aug 1, 1981·Experimental Cell Research·I YamaneY Minamoto
Dec 1, 1980·Cell·D Barnes, G Sato

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Citations

Jan 1, 1993·Cytotechnology·Z ChenY Chen
Jan 1, 1994·Cytotechnology·M Butler, A Christie
Apr 25, 2000·Brain Research. Developmental Brain Research·B M KivellJ H Miller
Sep 19, 2003·Brain Research Bulletin·Daan Ren, Joseph D Miller
Feb 27, 2001·Brain Research. Brain Research Protocols·B M KivellJ H Miller
Oct 20, 2005·Journal of Bioscience and Bioengineering·K SakaiH Fukuda
Oct 20, 2005·Journal of Bioscience and Bioengineering·Masahiro KawaharaTeruyuki Nagamune
Jul 12, 2007·Cell Division·Bradley S EllisonRobert J Boackle
Mar 27, 2012·Biotechnology and Bioengineering·Sohye KangRohini Deshpande
Jul 26, 2016·AMB Express·Andres Sánchez-KopperRalf Takors
Sep 2, 2006·Biotechnology and Bioengineering·Gina M AltarasGargi Maheshwari
Jul 9, 1999·Biotechnology and Bioengineering·A Christie, M Butler

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