Development of a serum-free system to expand dental-derived stem cells: PDLSCs and SHEDs

Journal of Cellular Physiology
Susan A TarleDarnell Kaigler

Abstract

Recently, extracted teeth have been identified as a viable source of stem cells for tissue regenerative approaches. Current expansion of these cells requires incorporation of animal sera; yet, a fundamental issue underlying cell cultivation methods for cell therapy regards concerns in using animal sera. In this study, we investigated the development of a chemically defined, serum-free media (K-M) for the expansion of human periodontal ligament stem cells (PDLSCs) and human stem cells from exfoliated deciduous teeth (SHEDs). Proliferation assays were performed comparing cells in serum-containing media (FBS-M) with cells cultured in four different serum-free medium and these demonstrated that in these medium, the cell proliferation of both cell types was significantly less than the proliferation of cells in FBS-M. Additional proliferation assays were performed using pre-coated fibronectin (FN) tissue culture plates and of the four serum-free medium, only K-M enabled PDLSCs and SHEDs to proliferate at higher rates than cells cultured in FBS-M. Next, alkaline phosphatase activity showed that PDLSCs and SHEDs exhibited similar osteogenic potential whether cultured in K-M or FBS-M, and, additionally, cells retained their multipotency...Continue Reading

References

Jan 1, 1979·Methods in Enzymology·J BottensteinR Wu
Jun 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·W L McKeehanR G Ham
Sep 1, 1991·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·S HaradaE Ogata
Feb 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·H MurakamiT Kano-Sueoka
Dec 1, 1980·Cell·D Barnes, G Sato
Jun 1, 1997·British Journal of Haematology·S A KuznetsovP G Robey
Apr 2, 1999·Science·M F PittengerD R Marshak
Nov 23, 2000·Proceedings of the National Academy of Sciences of the United States of America·S GronthosS Shi
Apr 30, 2003·Proceedings of the National Academy of Sciences of the United States of America·Masako MiuraSongtao Shi
Nov 25, 2003·Journal of Dental Research·S BatouliS Shi
Jul 19, 2005·Orthodontics & Craniofacial Research·S ShiS Gronthos
Oct 26, 2006·Journal of Dental Research·J J MaoS Shi
Mar 14, 2007·Stem Cells and Development·Neeraj Kumar SatijaR P Tripathi
Sep 14, 2007·Scandinavian Journal of Clinical and Laboratory Investigation·M Haack-SorensenJ Kastrup
Jan 23, 2009·Journal of Cellular Physiology·Naohisa WadaStan Gronthos
Mar 14, 2009·Biochemical and Biophysical Research Communications·Hideki AgataHideaki Kagami
Jul 16, 2009·Journal of Cellular and Molecular Medicine·Neeraj Kumar SatijaG U Gurudutta
Sep 4, 2009·Cytotherapy·Minoo Battiwalla, Peiman Hematti
Oct 14, 2009·Expert Opinion on Biological Therapy·Ofer SadanDaniel Offen

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Citations

Sep 5, 2013·In Vitro Cellular & Developmental Biology. Animal·Xuan WangLiangjun Zhong
Mar 24, 2016·Stem Cells International·Denise SalzigPeter Czermak
Apr 15, 2015·Stem Cells and Development·Petra HilkensIvo Lambrichts
Apr 11, 2015·Stem Cells International·Wenjun Zhu, Min Liang
May 3, 2014·Tissue Engineering. Part C, Methods·Oriana TrubianiNicoletta Zini
Nov 8, 2016·Stem Cells International·Athina Bakopoulou, Imad About
Dec 14, 2016·Journal of Tissue Engineering and Regenerative Medicine·Premjit ArpornmaeklongPrisana Pripatnanont
Oct 15, 2013·Cell Proliferation·S GottipamulaR N Seetharam
Dec 23, 2017·Frontiers in Physiology·Beatriz A Rodas-JuncoGeovanny I Nic-Can
Nov 16, 2019·Scientific Reports·Kengo NakajimaKotaro Tanimoto
Aug 29, 2020·International Journal of Molecular Sciences·Raquel Hernáez-MoyaNoelia Andollo
Apr 2, 2020·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·A JägerA Konermann

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