Development of a sheathless CE-ESI-MS interface

Electrophoresis
Akiyoshi HirayamaTomoyoshi Soga

Abstract

A sheath-flow interface is the most common ionization technique in CE-ESI-MS. However, this interface dilutes the analytes with the sheath liquid and decreases the sensitivity. In this study, we developed a sheathless CE-MS interface to improve sensitivity. The interface was fabricated by making a small crack approximately 2 cm from the end of a capillary column fixed on a plastic plate, and then covering the crack with a dialysis membrane to prevent metabolite loss during separation. A voltage for CE separation was applied between the capillary inlet and the buffer reservoir. Under optimum conditions, 52 cationic metabolite standards were separated and selectively detected using MS. With a pressure injection of 5 kPa for 15 s (ca. 1.4 nL), the detection limits for the tested compounds were between 0.06 and 1.7 μmol/L (S/N = 3). The method was applied to analysis of cationic metabolites extracted from a small number (12 000) of cancer cells, and the number of peaks detected was about 2.5 times higher than when using conventional sheath-flow CE-MS. Because the interface is easy to construct, it is cost-effective and can be adapted to any commercially available capillaries. This method is a powerful new tool for highly sensitive ...Continue Reading

References

Oct 30, 2003·Journal of Proteome Research·Tomoyoshi SogaTakaaki Nishioka
Oct 1, 1992·Analytical Chemistry·C W Whang, I C Chen
Mar 3, 2010·Electrophoresis·E Jane MaxwellDavid D Y Chen
Mar 20, 2010·Metabolomics : Official Journal of the Metabolomic Society·Masahiro SugimotoMasaru Tomita
Oct 12, 2012·Analytical and Bioanalytical Chemistry·Akiyoshi HirayamaTomoyoshi Soga
May 13, 2015·Electrophoresis·Michal CiborowskiAdam Kretowski
Jun 3, 2015·Electrophoresis·Juan Carlos García-CañaverasAgustín Lahoz
Nov 26, 2015·Electrophoresis·Can GulersonmezRawi Ramautar

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Citations

Sep 21, 2018·Electrophoresis·Rawi RamautarGerhardus J de Jong
Dec 18, 2019·Bioanalysis·Karen SegersAnn Van Eeckhaut
Aug 12, 2021·Analytical Methods : Advancing Methods and Applications·Bo LiuHong-Xu Chen
Oct 19, 2019·Analytical Chemistry·Carolina González-RianoCoral Barbas
Nov 9, 2019·Analytical Chemistry·Courtney J KristoffLisa A Holland

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