Development of a short-term human full-thickness skin organ culture model in vitro under serum-free conditions

Archives of Dermatological Research
Konrad Kleszczyński, T W Fischer

Abstract

Skin exerts a variety of important functions to maintain its integrity and viability. It can be used as an experimental ex vivo model to study wound healing, oxidative stress, skin aging, carcinogenesis as well as topical or "systemic" therapeutic intervention in vitro. This report aims to validate a serum-free human full-thickness skin organ culture model with regard to dependency of skin viability on culture duration and location of skin cross-section (1-5) from the outer (section 1) to the inner side (section 5) of a skin specimen (5 × 10 mm). Cultured skin was analyzed in time-dependent manner for structural damage (H&E staining) and 'balance' between proliferation (Ki67) and apoptosis [cleavage of caspase-3, lactate dehydrogenase (LDH), TUNEL]. First structural disturbances were observed at 48 h (section 3; middle part), increasing with prolonged culture time. Cleavage of caspase-3 and appearance of apoptotic [TUNEL(+)] cells showed significant increase at 72 h in sections 4 and 5, respectively. This correlated to increasing LDH release. Parallel analysis of proliferating [Ki67(+)] cells revealed simultaneous down-regulation within the first 48 h reaching complete absence of Ki67(+) cells at 72 h. These data define an accu...Continue Reading

References

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Citations

Sep 11, 2012·Archives of Dermatological Research·Antonio Peramo, Cynthia L Marcelo
Oct 15, 2013·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Konrad KleszczyńskiTobias W Fischer
Apr 27, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Tae-Kang KimAndrzej T Slominski
Aug 19, 2015·Ecotoxicology and Environmental Safety·Francesca SimoncelliAnna Fagotti
Apr 9, 2016·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·Francesca AbramoVincenzo Miragliotta
Dec 7, 2017·International Journal of Molecular Sciences·Stefan RiwaldtDaniela Grimm
Mar 28, 2018·Experimental Dermatology·Ewan A LanganRalf Paus
Jan 4, 2019·Journal of Applied Toxicology : JAT·Jitka VostálováAlena Rajnochová Svobodová
Aug 19, 2015·Experimental Dermatology·Ewan A LanganRalf Paus
Jun 25, 2019·Frontiers in Microbiology·Dora E Corzo-LeónDonna M MacCallum
Apr 3, 2021·Experimental Dermatology·Christoph H TrippPatrizia Stoitzner

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