Abstract
Skin exerts a variety of important functions to maintain its integrity and viability. It can be used as an experimental ex vivo model to study wound healing, oxidative stress, skin aging, carcinogenesis as well as topical or "systemic" therapeutic intervention in vitro. This report aims to validate a serum-free human full-thickness skin organ culture model with regard to dependency of skin viability on culture duration and location of skin cross-section (1-5) from the outer (section 1) to the inner side (section 5) of a skin specimen (5 × 10 mm). Cultured skin was analyzed in time-dependent manner for structural damage (H&E staining) and 'balance' between proliferation (Ki67) and apoptosis [cleavage of caspase-3, lactate dehydrogenase (LDH), TUNEL]. First structural disturbances were observed at 48 h (section 3; middle part), increasing with prolonged culture time. Cleavage of caspase-3 and appearance of apoptotic [TUNEL(+)] cells showed significant increase at 72 h in sections 4 and 5, respectively. This correlated to increasing LDH release. Parallel analysis of proliferating [Ki67(+)] cells revealed simultaneous down-regulation within the first 48 h reaching complete absence of Ki67(+) cells at 72 h. These data define an accu...Continue Reading
References
Jul 1, 1994·Journal of Dermatological Science·M P PhilpottT Kealey
Feb 24, 2001·Photochemistry and Photobiology·D L MitchellM G Lowery
Jul 26, 2003·The Journal of Investigative Dermatology·Kathi C Madison
Oct 7, 2003·Skin Pharmacology and Applied Skin Physiology·U-C HiplerP Elsner
Mar 2, 2006·The American Journal of Pathology·Kerstin FoitzikRalf Paus
Dec 22, 2006·Experimental Dermatology·Zhongfa LuRalf Paus
Jun 19, 2007·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Konrad KleszczyńskiAndrzej C Składanowski
Sep 8, 2007·The American Journal of Pathology·Eniko BodóRalf Paus
Dec 2, 2008·Experimental Dermatology·Ehrhardt ProkschJens-Michael Jensen
Jan 17, 2009·The American Journal of Pathology·Akira HachiyaRaymond E Boissy
Jan 27, 2009·Toxicology·Marijke A E MolHenk P Benschop
May 28, 2009·Experimental Dermatology·Meital Portugal-CohenYoram Milner
Nov 21, 2009·Experimental Dermatology·Burkhard PoeggelerRalf Paus
Dec 19, 2009·Annals of Biomedical Engineering·Antonio PeramoDavid C Martin
Oct 12, 2011·World Journal of Gastroenterology : WJG·Jian-Wen JiangShu-Sen Zheng
Dec 7, 2011·Journal of Conservative Dentistry : JCD·Hany Mohamed Aly AhmedDeepti Saini
Citations
Sep 11, 2012·Archives of Dermatological Research·Antonio Peramo, Cynthia L Marcelo
Oct 15, 2013·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Konrad KleszczyńskiTobias W Fischer
Aug 13, 2013·PloS One·Stefan TukajMichael Kasperkiewicz
Apr 27, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Tae-Kang KimAndrzej T Slominski
Aug 19, 2015·Ecotoxicology and Environmental Safety·Francesca SimoncelliAnna Fagotti
Nov 1, 2012·Journal of Pineal Research·Tobias W FischerDetlef Zillikens
Apr 9, 2016·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·Francesca AbramoVincenzo Miragliotta
Nov 27, 2014·Journal of Pineal Research·Konrad KleszczyńskiTobias W Fischer
Dec 3, 2014·European Journal of Cell Biology·Magnus S ÅgrenUrsula Mirastschijski
Dec 7, 2017·International Journal of Molecular Sciences·Stefan RiwaldtDaniela Grimm
Mar 28, 2018·Experimental Dermatology·Ewan A LanganRalf Paus
Jan 4, 2019·Journal of Applied Toxicology : JAT·Jitka VostálováAlena Rajnochová Svobodová
Aug 19, 2015·Experimental Dermatology·Ewan A LanganRalf Paus
Jun 25, 2019·Frontiers in Microbiology·Dora E Corzo-LeónDonna M MacCallum
Dec 15, 2020·Scientific Reports·Christin von MüllerSlavena Vylkova
Apr 3, 2021·Experimental Dermatology·Christoph H TrippPatrizia Stoitzner