Development of a sialic acid-containing hydrogel of poly[N-(2-hydroxypropyl) methacrylamide]: characterization and implantation study

Biomacromolecules
Stéphane WoerlyMarc Savasta

Abstract

This study describes the preparation and the characterization of poly[ N-(2-hydroxypropyl methacrylamide)] hydrogel with bulk-modified saccharidic portion of ganglioside GM 3 (Neu5Ac-alpha2,3-Gal-beta1,4-Glc). The 3'-sialyllactose is a bioactive epitope recognized by many cell surface receptors on viruses, bacteria, and human cells such as growth factor receptors. Acrylated 3'-sialyllactose was synthesized and incorporated into the macromolecular network of hydrogels by free radical cross-linking copolymerization. Fluorescence techniques coupled to confocal laser scanning microscopy was employed to characterize the binding and accessibility of the sialyl group in the polymer network by using a monoclonal antibody against GM 3 and the lectin wheat germ agglutinin. The morphology of the network was examined by scanning electron microscopy and confocal microscopy to image the gel morphology. The water content of sialyllactosyl-HPMA hydrogel compared to unmodified gel was characterized by swelling measurements and thermogravimetry. A preliminary implantation study in rat brain was performed to examine the biofunctionality of the sialyllactosyl hydrogel using an experimental model of Parkinson's disease.

References

Jan 1, 1994·Methods in Enzymology·C A LaferrièreR Roy
Jan 1, 1993·Journal of Neuroscience Research·P F MoskowitzM M Oblinger
Aug 1, 1998·Journal of Biomaterials Science. Polymer Edition·S WoerlyE Syková
Sep 7, 2000·The Journal of Comparative Neurology·T D PalmerF H Gage
Jan 5, 2002·Advanced Drug Delivery Reviews·Allan S Hoffman
Jan 18, 2002·The Journal of Biological Chemistry·Erik A MiljanEric G Bremer
Feb 19, 2002·Chemical Reviews·Eric E. SimanekChi-Huey Wong
Mar 19, 2002·Progress in Neurobiology·Lukas Sommer, Mahendra Rao
Jul 9, 2003·Annals of the New York Academy of Sciences·Stanley Fahn
Jan 6, 2004·Journal of Neuroscience Research·Stéphane WoerlyAraceli Espinosa-Jeffrey
Jan 28, 2004·Annual Review of Pharmacology and Toxicology·D Chichung LieFred H Gage
Mar 30, 2004·Biomaterials·Jamie OstrohaN Dan
May 19, 2005·Chemical Communications : Chem Comm·Sébastien FortHugues Driguez
May 9, 2006·The Journal of Comparative Neurology·Tsu-Wei WangJack M Parent
Dec 15, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·John J OhabS Thomas Carmichael
Feb 24, 2007·Glycobiology·Satoshi UemuraJin-Ichi Inokuchi
Jun 15, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Matthias ErschbamerLars Olson

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Citations

Mar 9, 2012·Regenerative Medicine·Sárka Kubinová, Eva Syková
Sep 29, 2011·Journal of Biomedical Materials Research. Part a·Sárka KubinováEva Syková
Jul 19, 2012·Journal of Cellular and Molecular Medicine·Ana Paula PêgoEva Sykova
Jul 22, 2010·Angewandte Chemie·Katrin KnopUlrich S Schubert
Jul 1, 2015·International Journal of Biological Macromolecules·Marilia M HornAna Maria de Guzzi Plepis
Mar 7, 2019·Chemistry, an Asian Journal·Preeti Madhukar ChaudharyRaghavendra Kikkeri
Jun 21, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xian LiXiaoxuan Ma
Mar 6, 2021·Biomaterials Science·Himadri Shekhar RoyDeepa Ghosh

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