Development of a silver in situ hybridisation based assay for the determination of ploidy status in molar pregnancy diagnosis

Pathology
Laurence A GaleaDavid S Williams

Abstract

The aim of this study was to establish a scoring method for ploidy analysis using silver in situ hybridisation (SISH) with a chromosome 17 centromere probe. SISH was performed using the Ventana chromosome 17 centromere probe on sections from formalin fixed, paraffin embedded archival cases of complete hydatidiform moles, partial hydatidiform moles and hydropic products of conception with previously established ploidy status (determined by flow cytometry or karyotyping). In order to determine ploidy status, a scoring method was developed based on both the average number of signals per nucleus (ASN) and the percentage of nuclei with three signals (N3S), enumerated in 50 villous cytotrophoblastic and/or stromal cells. The results of four independent observers were compared individually and collectively with previously established ploidy status. There was a highly statistically significant difference between diploid and triploid gestations for ASN (1.86 ± 0.13 and 2.70 ± 0.16 respectively, Student t-test, p < 0.0001) and for N3S (1.14 ± 1.65 and 71.59 ± 14.25 respectively, Student t-test, p < 0.0001). The sensitivity and specificity of the SISH-based assay was 99.1% and 100% respectively for ASN, and 100% and 100% respectively for ...Continue Reading

References

Jul 1, 1978·Lancet·P A JacobsI M Newlands
May 1, 1991·American Journal of Obstetrics and Gynecology·S D LawlerJ Dent
Apr 1, 1982·Cancer Genetics and Cytogenetics·S D LawlerM W Evans
Jul 1, 1993·Journal of Clinical Pathology·A J HowatR A Williams
Dec 5, 1996·The New England Journal of Medicine·R S Berkowitz, D P Goldstein
May 7, 1999·Molecular Diagnosis : a Journal Devoted to the Understanding of Human Disease Through the Clinical Application of Molecular Biology·K A BellD G Leonard
Jul 13, 2000·Lancet·M J SecklE S Newlands
Jul 19, 2001·Cancer Investigation·J S Ross, B J McKenna
Oct 18, 2001·International Journal of Gynecological Pathology : Official Journal of the International Society of Gynecological Pathologists·D R Genest
Nov 25, 2003·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Caroline Y L LaiAnnie N Y Cheung
Jul 4, 2006·Obstetrics and Gynecology·John T Soper
Feb 28, 2008·The American Journal of Surgical Pathology·Carlo BifulcoPei Hui
Apr 23, 2008·American Journal of Clinical Pathology·Robin D LeGalloKristen A Atkins
Jan 1, 2007·Archives of Pathology & Laboratory Medicine·Antonio C WolffUNKNOWN American Society of Clinical Oncology/College of American Pathologists
Jun 28, 2011·The Journal of Molecular Diagnostics : JMD·Sarah ChiangA John Iafrate
Jan 2, 2013·Archives of Pathology & Laboratory Medicine·Larissa V FurtadoKatherine B Geiersbach

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