Development of a simple and high-throughput method for detecting aflatoxins production in culture media

Letters in Applied Microbiology
Francesca DegolaFrancesco M Restivo

Abstract

To develop a simple, high-throughput and inexpensive procedure to detect and quantify aflatoxins into the culture media of growing mycelia. Fungal conidia (Aspergillus flavus) were inoculated into the wells of a microplate containing 200 μl of different formulations of coconut-derived liquid medium. Time-dependent production of aflatoxins in the culture media was evaluated by a procedure relying on the UV-induced fluorescence emission by the toxin, using a microplate reader. These data were validated by comparison with the outputs of a conventional HPLC-based procedure. Determinations of aflatoxin concentration, according to the fluorimetric procedure, were performed either by withdrawing samples from the plates or by direct 'in situ' readings, the latter method reinforcing the high-throughput feature of the procedure. Fluorescence enhancers (cyclodextrins) did not ameliorate the sensitivity of the procedure to low concentrations of the toxin into the medium. The efficacy of the procedure was also validated by testing the effect on toxin yield of adding an antioxidant agent (α-lipoic acid) to the medium. We give evidence that our improved procedure is reliable and suitable to analyse aflatoxin accumulation time course in coconu...Continue Reading

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Mar 23, 2011·International Journal of Food Microbiology·Francesca DegolaFrancesco M Restivo

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Citations

Feb 24, 2015·International Journal of Food Microbiology·Francesca DegolaTiziana Lodi
Jan 7, 2016·Journal of Food Protection·Larry R Beuchat, David A Mann
Nov 30, 2019·Toxins·Stephen Abiola AkinolaMulunda Mwanza
Jan 5, 2019·World Journal of Microbiology & Biotechnology·Modupeade C AdetunjiMulunda Mwanza
Apr 3, 2020·Environmental Science and Pollution Research International·Giorgio SpadolaDominga Rogolino
Feb 9, 2021·Frontiers in Microbiology·Nancy NleyaMulunda Mwanza
Sep 10, 2019·Journal of Agricultural and Food Chemistry·Jennifer BartoliMauro Carcelli

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