Development of a simple device for dispersive liquid-liquid microextraction with lighter than water organic solvents: isolation and enrichment of glycyrrhizic acid from licorice

Analytica Chimica Acta
Payman HashemiAli Reza Ghiasvand

Abstract

A simple device was developed that makes the use of lighter than water organic solvents feasible in dispersive liquid-liquid microextraction (DLLME) method. In the ordinary DLLME, the fact that a heavier than water organic solvent must be used, to be sedimented at the conical bottom of a centrifuge tube, limits the applications of this method in some extent. In the developed method, a glass tube with a narrow neck is inserted inside the centrifuge tube. After phase separation, the organic solvent is accumulated in the narrow neck of the device and therefore, can be simply collected by a micro-syringe. The DLLME method with the proposed device was tested for the enrichment of glycyrrhizic acid from aqueous extracts of licorice before analysis by a HPLC method. n-Hexanol and acetone were used as the organic and disperser phases, respectively. Effects of pH, salt concentration and phase volumes on the extraction of the analyte were optimized using a central composite (response surface) design. Under the optimized conditions (i.e. pH 1.3, ionic strength 0.2 mol L(-1), n-hexanol 140 microL and acetone 0.8 mL) an extraction recovery of 104.1 (+/-5.1)% and an enrichment factor of 54 were obtained. The proposed method was successfully ...Continue Reading

Citations

Sep 13, 2011·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Payman HashemiAli Reza Ghiasvand
Nov 22, 2013·Analytical and Bioanalytical Chemistry·Mohammad Saraji, Malihe Khalili Boroujeni
Jan 29, 2016·Analytical Chemistry·Liang GuoHian Kee Lee
Nov 28, 2012·Journal of Separation Science·Mohammad Reza HadjmohammadiPanteha Shakeri
Jun 27, 2015·Forensic Science International : Synergy·Muhammad ImranHafiz Faisal Usman
Apr 16, 2020·Phytochemical Analysis : PCA·Alina DiuzhevaVasil Andruch
Jun 30, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Carmela ConidiAlfredo Cassano
Jul 28, 2021·Journal of the Science of Food and Agriculture·Marieh MardaniRazieh Niazmand

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