Development of a split SNAP-tag protein complementation assay for visualization of protein-protein interactions in living cells

The Analyst
Masayasu MieEiry Kobatake


A split SNAP-tag protein complementation assay was developed for visualization of protein-protein interactions in living cells. Split SNAP-tagμs, fragments of divided SNAP-tag between amino acid residues 91 and 92, were fused to proteins that can interact with each other. After incubation with a fluorescent SNAP-tag substrate, cells that expressed split SNAP-tag fusion proteins generated fluorescent signals when these proteins interacted. Moreover, by combination with the split CLIP-tag (SNAP-tag mutant), simultaneous labeling was achieved. This split SNAP-tag labeling method should be a useful tool for visualization of protein-protein interaction processes.


Nov 21, 2018·Methods and Applications in Fluorescence·Sergiy V Avilov, Nataliia Aleksandrova
Dec 11, 2019·International Journal of Molecular Sciences·Tiphaine Péresse, Arnaud Gautier
Oct 13, 2019·Nature Protocols·Ahmad Fawzi HussainStefan Barth


Jun 15, 2000·Proceedings of the National Academy of Sciences of the United States of America·C T RollinsT Clackson
Dec 7, 2002·Nature Biotechnology·Antje KepplerKai Johnsson
Mar 31, 2005·Journal of the American Chemical Society·Laura A BanaszynskiThomas J Wandless
Nov 16, 2005·Biochemistry·Qingming FangAnthony E Pegg
Apr 28, 2006·Protein Engineering, Design & Selection : PEDS·Thomas GronemeyerKai Johnsson
Sep 5, 2006·Current Opinion in Chemical Biology·Elizabeth A Jares-Erijman, Thomas M Jovin
Jan 27, 2007·Current Opinion in Biotechnology·Tarik F MassoudSanjiv S Gambhir
Mar 7, 2007·The Journal of Cell Biology·Lars E T JansenDon W Cleveland
Jun 30, 2007·Nature Reviews. Drug Discovery·Stephen W MichnickEduard Stefan
Feb 23, 2008·Chemistry & Biology·Arnaud GautierKai Johnsson
Aug 5, 2008·Chembiochem : a European Journal of Chemical Biology·Judith Müller, Nils Johnsson
Nov 13, 2008·Applied Biochemistry and Biotechnology·Azadeh SeidiEiry Kobatake
Mar 6, 2010·Journal of the American Chemical Society·Duangkhae SrikunChristopher J Chang
Apr 9, 2011·Journal of the American Chemical Society·Toru KomatsuYasuteru Urano
Nov 22, 2011·Journal of the American Chemical Society·Sarah A SlavoffAlice Y Ting

Related Concepts

Chimeric Proteins, Recombinant
Fluorescent stain
Protein-Protein Interaction
HeLa Cells
Protein Interaction Maps
Amino Acid [EPC]

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Sexual Dimorphism in Neurodegeneration

There exist sex differences in neurodevelopmental and neurodegenerative disorders. For instance, multiple sclerosis is more common in women, whereas Parkinson’s disease is more common in men. Here is the latest research on sexual dimorphism in neurodegeneration

HLA Genetic Variation

HLA genetic variation has been found to confer risk for a wide variety of diseases. Identifying these associations and understanding their molecular mechanisms is ongoing and holds promise for the development of therapeutics. Find the latest research on HLA genetic variation here.

Super-resolution Microscopy

Super-resolution microscopy is the term commonly given to fluorescence microscopy techniques with resolutions that are not limited by the diffraction of light. Here are the latest discoveries pertaining to super-resolution microscopy.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.

Brain Lower Grade Glioma

Low grade gliomas in the brain form from oligodendrocytes and astrocytes and are the slowest-growing glioma in adults. Discover the latest research on these brain tumors here.

CD4/CD8 Signaling

Cluster of differentiation 4 and 8 (CD8 and CD8) are glycoproteins founds on the surface of immune cells. Here is the latest research on their role in cell signaling pathways.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.