Development of a split SNAP-tag protein complementation assay for visualization of protein-protein interactions in living cells

The Analyst
Masayasu MieEiry Kobatake

Abstract

A split SNAP-tag protein complementation assay was developed for visualization of protein-protein interactions in living cells. Split SNAP-tagμs, fragments of divided SNAP-tag between amino acid residues 91 and 92, were fused to proteins that can interact with each other. After incubation with a fluorescent SNAP-tag substrate, cells that expressed split SNAP-tag fusion proteins generated fluorescent signals when these proteins interacted. Moreover, by combination with the split CLIP-tag (SNAP-tag mutant), simultaneous labeling was achieved. This split SNAP-tag labeling method should be a useful tool for visualization of protein-protein interaction processes.

References

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Related Concepts

Chimeric Proteins, Recombinant
Fluorescent stain
Protein-Protein Interaction
Mutant
Fluorochromes
HeLa Cells
Fragments
Protein Interaction Maps
Assay
Amino Acid [EPC]

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