PMID: 2124701Dec 1, 1990Paper

Development of a stable Leishmania expression vector and application to the study of parasite surface antigen genes

Proceedings of the National Academy of Sciences of the United States of America
J H LeBowitzS Beverley

Abstract

Trypanosomatid protozoan parasites cause several important tropical diseases and have been a fertile ground for the discovery of molecular paradigms such as trans-splicing and RNA editing. Transfection-based methods for the study of these organisms have recently been developed, and we have now designed an expression vector, pX, which contains only 2.3 kilobases of Leishmania DNA and can be stably transfected with high efficiency. Genes encoding Escherichia coli beta-galactosidase or a Leishmania amazonensis protective membrane glycoprotein (GP46A/M-2) were inserted into the pX expression site and transfected into Leishmania major, where they directed the synthesis of high levels of mRNAs formed by 5' and 3' processing events occurring predominantly at the sites used by the normal transcripts. Colony assays and immunoblot analysis showed that both proteins were produced; enzymatically active beta-galactosidase comprised approximately 1% of total protein. Sizes of the GP46A protein synthesized in transfected L. major or L. amazonensis were similar and differed from the predominant L. amazonensis GP46, suggesting that the GP46A gene may encode a variant GP46 family member. Because these vectors function efficiently in pathogenic s...Continue Reading

References

Nov 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·K L LohmanD McMahon-Pratt
Jun 9, 1989·Science·V Bellofatto, G A Cross
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A Laban, D F Wirth
Jan 1, 1986·Annual Review of Biochemistry·P Borst
Nov 1, 1982·Molecular and Biochemical Parasitology·D M Pratt, J R David

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Citations

Dec 21, 2010·Amino Acids·Ehud InbarClaudio A Pereira
May 1, 1991·Molecular and Biochemical Parasitology·C M CoburnS M Beverley
Apr 1, 1992·Molecular and Biochemical Parasitology·J H LeBowitzS M Beverley
Jan 1, 1992·Molecular and Biochemical Parasitology·D McMahon-PrattS M Beverley
Nov 1, 1993·Molecular and Biochemical Parasitology·D J Freedman, S M Beverley
Feb 1, 1994·Molecular and Biochemical Parasitology·A E SouzaJ C Mottram
Feb 1, 1994·Molecular and Biochemical Parasitology·J R Webb, W R McMaster
May 1, 1994·Molecular and Biochemical Parasitology·B PapadopoulouM Ouellette
Mar 1, 1994·Molecular and Biochemical Parasitology·M ArgamanM Shapira
Mar 1, 1994·Molecular and Biochemical Parasitology·D H ChenY Suyama
Jan 1, 1996·Molecular and Biochemical Parasitology·B DallagiovannaS Castanys
Feb 1, 1996·Molecular and Biochemical Parasitology·S R UlianaL M Floeter-Winter
Jul 1, 1996·Molecular and Biochemical Parasitology·R ArrebolaD González-Pacanowska
Nov 25, 1996·Molecular and Biochemical Parasitology·M WieseP Overath
Feb 11, 1994·Gene·M V de Arruda, P Matsudaira
Mar 22, 2005·International Journal for Parasitology·Santiago Martínez-CalvilloPeter J Myler
Feb 19, 2002·International Journal for Parasitology·Valeska de Andrade Stempliuk, Lucile Maria Floeter-Winter
Jun 6, 2000·Molecular and Biochemical Parasitology·S Goyard, S M Beverley
Nov 23, 2000·Molecular and Biochemical Parasitology·I BenzelM Wiese
Jun 23, 2001·Molecular and Biochemical Parasitology·S C RobertsB Ullman
Mar 19, 2002·Molecular and Biochemical Parasitology·Emmanuel TetaudAlan H Fairlamb
Sep 1, 1997·Molecular and Biochemical Parasitology·G BartJ C Mottram
Nov 6, 1998·Molecular and Biochemical Parasitology·J J McCoyM E Wilson
Jan 8, 1999·Molecular and Biochemical Parasitology·C ClaytonL Vanhamme
Dec 10, 1999·Molecular and Biochemical Parasitology·N Downey, J E Donelson
Dec 10, 1999·Molecular and Biochemical Parasitology·N Downey, J E Donelson
Dec 29, 1999·Molecular and Biochemical Parasitology·C E ClaytonS M Beverley
Aug 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·A CruzS M Beverley
Jun 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·X Liu, K P Chang
Jun 7, 1994·Proceedings of the National Academy of Sciences of the United States of America·L D SibleyI R Niesman
Oct 24, 1995·Proceedings of the National Academy of Sciences of the United States of America·R G TitusS M Beverley
Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·J C MottramG H Coombs
Jul 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·B S Crabb, A F Cowman

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