Development of a supercritical fluid chromatography-tandem mass spectrometry method for the determination of lacidipine in beagle dog plasma and its application to a bioavailability study

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Yajie GengTianhong Zhang

Abstract

A simple, novel, rapid and sensitive supercritical fluid chromatography-tandem mass spectrometry (SFC-MS/MS) method was developed and validated for the determination of lacidipine in beagle dog plasma with nimodipine as internal standard. The method involved a simple liquid-liquid extraction method with tert-butyl methyl ether. The analytes were analyzed on an Acquity UPC(2) with a HSS C18 SB column (3mm×100mm, 1.8μm) set at 50°C. The mobile phase was carbon dioxide (≥99.99%) and methanol (92:8, v/v) at a flow rate of 2ml/min, the compensation solvent was methanol with 2% formic acid at a flow rate of 0.2ml/min and a total analysis time of 1.5min for each sample. The multiple reaction-monitoring mode was used for quantification of ion transitions at m/z 473.32→354.10 and 419.00→343.10 for lacidipine and internal standard, respectively. The linearity range of proposed method was 0.10-100ng/ml) (r(2)≥0.9990). The intra- and inter-day precision values were less than 15% and accuracy was from -0.83% to 3.27% at all quality control levels. The proposed method was successfully applied to a pharmacokinetic study of lacidipine in beagle dogs.

References

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Citations

Jan 22, 2015·Journal of Chromatography. a·Eric Lesellier, Caroline West
Aug 11, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Rujie YangTianhong Zhang
Oct 11, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Mohammad Mahdi MoeinMohamed Abdel-Rehim
May 5, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jing GuoBei Fan

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