Development of a tandem time-of-flight mass spectrometer with an electrospray ionization ion source

Journal of Mass Spectrometry : JMS
Hirofumi NagaoMichisato Toyoda

Abstract

A new tandem time-of-flight mass spectrometer with an electrospray ionization ion source 'ESI-TOF/quadTOF' was designed and constructed to achieve the desired aim of structural elucidation via high-energy collision-induced dissociation (CID), and the simultaneous detection of all fragment ions. The instrument consists of an orthogonal acceleration-type ESI ion source, a linear TOF mass spectrometer, a collision cell, a quadratic-field ion mirror and a microchannel plate detector. High-energy CID spectra of doubly protonated angiotensin II and bradykinin were obtained. Several fragment ions such as a-, d-, v- and w-type ions, characteristic of high-energy CID, were clearly observed in these spectra. These high-energy CID fragment ions enabled confirmation of the complete sequence, including leucine-isoleucine determinations. It was demonstrated that high-energy CID of multiply protonated peptides could be achieved in the ESI-TOF/quadTOF.

References

Jan 1, 1992·Annual Review of Biochemistry·K Biemann
Feb 1, 1992·Biological Mass Spectrometry·B L Schwartz, M M Bursey
Oct 1, 1988·Biomedical & Environmental Mass Spectrometry·K Biemann
Nov 1, 1984·Biomedical Mass Spectrometry·P Roepstorff, J Fohlman
Nov 1, 1995·Analytical Chemistry·D J BeussmanC G Enke
Oct 1, 1996·Journal of Mass Spectrometry : JMS·T Vaisar, J Urban
Mar 14, 2003·Nature·Ruedi Aebersold, Matthias Mann
Apr 11, 2003·Journal of the American Society for Mass Spectrometry·Fong-Fu Hsu, John Turk
Jun 28, 2003·Analytical and Bioanalytical Chemistry·Detlev SuckauArmin Holle
Jan 13, 2006·Methods in Enzymology·J Mitchell Wells, Scott A McLuckey
Feb 10, 2009·Combinatorial Chemistry & High Throughput Screening·Ernst Pittenauer, Günter Allmaier
May 9, 2009·European Journal of Mass Spectrometry·Kenichi IwamotoMichisato Toyoda
Jun 1, 1996·Journal of the American Society for Mass Spectrometry·M M CorderoR J Cotter
Sep 1, 1993·Journal of the American Society for Mass Spectrometry·M A SeeterlinC G Enke
Nov 1, 1994·Journal of the American Society for Mass Spectrometry·K M Downard, K Biemann

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Citations

Jun 1, 2013·Rapid Communications in Mass Spectrometry : RCM·Hubert GnaserJiro Matsuo
May 15, 2015·Mass Spectrometry Reviews·Anna RadionovaPeter J Derrick
Nov 17, 2010·Rapid Communications in Mass Spectrometry : RCM·Julie AllegrandOlivier Laprévote
Feb 26, 2020·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Akihiro KitashojiTomoyuki Yatsuhashi

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