Development of a targeted adductomic method for the determination of polycyclic aromatic hydrocarbon DNA adducts using online column-switching liquid chromatography/tandem mass spectrometry.

Rapid Communications in Mass Spectrometry : RCM
Rajinder SinghPeter B Farmer

Abstract

Human exposure to polycyclic aromatic hydrocarbons (PAHs) from sources such as industrial or urban air pollution, tobacco smoke and cooked food is not confined to a single compound, but instead to mixtures of different PAHs. The interaction of different PAHs may lead to additive, synergistic or antagonistic effects in terms of DNA adduct formation and carcinogenic activity resulting from changes in metabolic activation to reactive intermediates and DNA repair. The development of a targeted DNA adductomic approach using liquid chromatography/tandem mass spectrometry (LC/MS/MS) incorporating software-based peak picking and integration for the assessment of exposure to mixtures of PAHs is described. For method development PAH-modified DNA samples were obtained by reaction of the anti-dihydrodiol epoxide metabolites of benzo[a]pyrene, benzo[b]fluoranthene, dibenzo[a,l]pyrene (DB[a,l]P) and dibenz[a,h]anthracene with calf thymus DNA in vitro and enzymatically hydrolysed to 2'-deoxynucleosides. Positive LC/electrospray ionisation (ESI)-MS/MS collision-induced dissociation product ion spectra data showed that the majority of adducts displayed a common fragmentation for the neutral loss of 116 u (2'-deoxyribose) resulting in a major pr...Continue Reading

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Citations

Feb 28, 2013·Chemical Reviews·Natalia TretyakovaSrikanth Kotapati
Jul 26, 2012·Chemical Research in Toxicology·Natalia TretyakovaGregory Janis
Jan 21, 2014·Chemical Research in Toxicology·Silvia BalboPeter W Villalta
Nov 26, 2014·Analytical Chemistry·Bernhard H MonienChristophe Chesné
Oct 29, 2014·Chemical Research in Toxicology·Henrik CarlssonMargareta Törnqvist
Aug 26, 2015·MicrobiologyOpen·Robert A KanalyNatsuko Hamamura
Feb 9, 2017·Marine Environmental Research·Daniela M PampaninMagne O Sydnes
Dec 13, 2016·Journal of Applied Genetics·Błaszczyk Ewa, Mielżyńska-Švach Danuta
May 17, 2019·High-throughput·Jingshu Guo, Robert J Turesky
Nov 29, 2013·Mass Spectrometry Reviews·Ed Dudley, Liz Bond
Aug 29, 2017·International Journal of Molecular Sciences·Peter W Villalta, Silvia Balbo
Apr 7, 2021·Rapid Communications in Mass Spectrometry : RCM·Poguang WangRoger W Giese
Oct 5, 2017·Analytical Chemistry·Jingshu GuoRobert J Turesky

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