Development of a thrombin generation test in cultured endothelial cells: Evaluation of the prothrombotic effects of antiphospholipid antibodies

Thrombosis Research
Paul BilloirVéronique Le Cam Duchez

Abstract

Circulating antiphospholipid antibodies (APL) induce vascular injury and endothelial dysfunction, which are associated with thrombotic events and/or fetal loss. We developed a model in which calibrated automated thrombin generation (CAT) is carried out in wells lined with cultured endothelial cells. Then we investigated how far b2GP1 antibodies provoked thrombin generation (TG) enhancing effects in these cells and/or in blood platelets. Thrombin generation induced by different concentrations of tissue factor and different levels of endothelial aortic cell confluence was investigated by calibrated automated thrombogram. Endothelial cells were incubated with the purified anti-β2glycoprotein I antibodies of patients with antiphospholipid syndrome (APS). Platelet free plasma and platelet rich plasma were used to study thrombin generation in endothelial cells and platelet reactivity, respectively. Endothelial cell confluence was negatively correlated with thrombin generation which was dependent on the concentration of APL incubated. Activation of endothelial cells with APL significantly increased thrombin generation triggered by PFP. Triggering by PRP increased thrombinogram parameters. Moreover, anti-β2glycoprotein I antibodies inc...Continue Reading

Citations

Jun 22, 2021·Frontiers in Medicine·Paul BilloirVéronique Le Cam Duchez
Jun 28, 2021·La Revue de médecine interne·P BilloirY Benhamou
Jul 15, 2021·Seminars in Thrombosis and Hemostasis·Gábor SzabóJános Kappelmayer
Oct 21, 2021·Clinica Chimica Acta; International Journal of Clinical Chemistry·Fiston KasongaPaul Billoir

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