Development of a time-resolved fluoroimmunoassay (TR-FIA) for testosterone: measurement of serum testosterone concentrations after testosterone treatment in the rainbow trout (Oncorhynchus mykiss)

General and Comparative Endocrinology
H YamadaMunehico Iwata

Abstract

A sensitive time-resolved fluoroimmunoassay (TR-FIA) for testosterone was developed, and the assay system was used for measuring serum testosterone concentrations in rainbow trout. Testosterone-3-(O-carboxymethyl)oxime-bovine serum albumin (T-3-CMO-BSA) was immobilized by physical adsorption to the wells of microtiter plates. A competitive assay using two antibodies was performed among T-3-CMO-BSA in the solid-phase, unknown amounts of testosterone, testosterone antibodies, and europium labeled secondary antibodies, followed by measurements using a time-resolved fluorometer (DELFIA system). The TR-FIA had a sensitivity of 0.075 pg/50 microliters sample (1.5 pg/ml), and the range of the assay system was between 1.5 pg/ml and 25 ng/ml. The intra- and interassay coefficients of variation for the testosterone TR-FIA were satisfactorily low, and were between 1.62 and 6.38% and 2.96 and 8.29%, respectively. The assay system was applied to measure the serum testosterone concentrations after an injection of testosterone dissolved in saline, propyleneglycol, or coconut oil. Among the three solvents, the coconut oil group showed continuously high serum testosterone level. In contrast, the saline and propyleneglycol groups had maximum con...Continue Reading

References

Nov 1, 1959·The Journal of Clinical Investigation·S A BERSON, R S YALOW

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Citations

Aug 25, 2010·Proceedings of the National Academy of Sciences of the United States of America·Katsuhisa UchidaMasumi Nozaki
Jun 27, 2012·Proceedings of the National Academy of Sciences of the United States of America·Sandip BhattaTakeshi Miura
Jan 12, 2010·PloS One·Yuzo YamamotoHiroshi Ueda
Oct 7, 2011·Reproduction : the Official Journal of the Society for the Study of Fertility·Chiemi MiuraTakeshi Miura
May 3, 2006·Proceedings of the National Academy of Sciences of the United States of America·Takeshi MiuraChiemi Miura
May 15, 2007·General and Comparative Endocrinology·Christian G WestringPhillip B Danielson
Aug 25, 2004·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Masafumi AmanoKunio Yamamori
Nov 26, 2015·Zoological Letters·Maiko KawajiriKazunori Yamahira
Sep 13, 2012·General and Comparative Endocrinology·Jaim SivanAvi Rosenstrauch
Feb 18, 2016·Scientific Reports·Hiroshi UedaMasahiko Watanabe
Jul 13, 2004·Journal of Biological Rhythms·Md Saydur RahmanYoung-Don Lee
Jun 3, 2017·Biology of Reproduction·Mana SatoGoro Yoshizaki
Sep 28, 2004·Journal of Experimental Zoology. Part A, Comparative Experimental Biology·Akihiro TakemuraMasaya Morita
May 15, 2001·The Journal of Experimental Zoology·M AmanoK Aida
Sep 22, 2018·General and Comparative Endocrinology·Mitsuo NyujiKoichi Okuzawa
Jan 6, 2000·General and Comparative Endocrinology·C H SummersK J Renner
Sep 13, 2002·General and Comparative Endocrinology·Masafumi AmanoKunio Yamamori

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