Development of a transposon mutagenesis system in the oral spirochete Treponema denticola.

Applied and Environmental Microbiology
Yu YangChunhao Li

Abstract

Here, we report successful transposon mutagenesis in the oral spirochete Treponema denticola. A modified Himar1 transposon, including a new antibiotic selection cassette for T. denticola, generated mutations affecting cell division, transport, and chemotaxis, among other processes. This random mutagenesis system should facilitate research on the biology and pathogenesis of this spirochete, which is associated with human periodontal diseases.

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Sep 13, 2011·Journal of Comparative Pathology·Y JinD Lin
Aug 10, 2010·Journal of Microbiological Methods·M Paula Goetting-Minesky, J Christopher Fenno
Dec 21, 2013·Veterinary Microbiology·Luke J MappleyMartin J Woodward
Mar 19, 2011·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M B Visser, R P Ellen
Dec 20, 2015·Applied and Environmental Microbiology·Kurni Kurniyati, Chunhao Li
Apr 19, 2015·Applied and Environmental Microbiology·Yuebin LiHui Wu
Jan 22, 2015·Journal of Bacteriology·Jiang BianChunhao Li

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