Development of a Two Triplex Real-Time Polymerase Chain Reaction for Rapid Detection of Six Carbapenemase Genes in Enterobacteriaceae

Osong Public Health and Research Perspectives
Ji Ae ChoiKwang Jun Lee

Abstract

Carbapenem resistance is a serious clinical and public health threat. Carbapenemase can confer carbapenem resistance, and most carbapenemase genes are plasmid encoded so resistance can easily spread. In this study, we aimed to develop a novel system based on the TaqMan platform for the rapid detection of 6 clinically prevalent carbapenemase genes: Klebsiella pneumoniae carbapenemase, New Delhi metallo-β-lactamase, oxacillinase, imipenem-hydrolyzing, Verona integron-encoded metallo-β-lactamase, and Guiana extended-spectrum β-lactamase. The triplex assay was verified by testing genomic DNA of 6 carbapenemase-producing Klebsiella pneumoniae. It was validated with a blinded panel of 310 Enterobacteriaceae isolates, including 225 carbapenemase-producers and 85 non-producers, by direct colony triplex real-time polymerase chain reaction (PCR). The real-time PCR was performed using the ABI 7500 fast instrument (Applied Biosystems, CA, USA) and specific primers for each carbapenemase target were designed to include modified peptide-nucleic acid oligonucleotides. No amplification was detected among the negative samples. The result showed 100% concordance with the genotypes previously identified. The entire assay, including DNA extraction...Continue Reading

References

Jun 2, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A Ray, B Nordén
Feb 20, 2007·International Journal of Antimicrobial Agents·Matthew E Falagas, Ioannis A Bliziotis
Feb 23, 2008·The Lancet Infectious Diseases·Johann D D Pitout, Kevin B Laupland
Jun 27, 2009·Journal of Medical Microbiology·David LandmanJohn Quale
Nov 6, 2009·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·L Silvia Munoz-Price, John P Quinn
Feb 26, 2010·Journal of Clinical Microbiology·Kenneth S Thomson
Jul 28, 2010·Antimicrobial Agents and Chemotherapy·Brandon KitchelJean B Patel
Mar 15, 2011·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Iruka N OkekeKatarina Nordqvist
Jun 10, 2011·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Neil GuptaAlexander J Kallen
Oct 18, 2011·Emerging Infectious Diseases·Patrice NordmannLaurent Poirel
Nov 26, 2011·Journal of Clinical Microbiology·Delphine GirlichPatrice Nordmann
Apr 18, 2012·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·R CantónUNKNOWN European Network on Carbapenemases
Apr 18, 2012·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·P NordmannUNKNOWN European Network on Carbapenemases
Jul 13, 2012·Journal of Clinical Microbiology·Martin KaaseSören G Gatermann
Apr 3, 2013·Cleveland Clinic Journal of Medicine·Federico Perez, David Van Duin
Apr 20, 2013·The Analyst·Shuang GuoGuo-Jun Zhang
Jun 17, 2014·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·P Nordmann, L Poirel
Jul 11, 2014·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·E YusufD Piérard
Dec 2, 2014·Nature Reviews. Microbiology·Jessica M A BlairLaura J V Piddock
Jan 7, 2015·Saudi Journal of Biological Sciences·Sibhghatulla ShaikhMohammad Amjad Kamal
Oct 22, 2015·Emerging Infectious Diseases·Latania K LoganUNKNOWN Prevention Epicenters Program
Mar 30, 2016·Antimicrobial Agents and Chemotherapy·Laurent DortetThierry Naas
May 25, 2016·Antimicrobial Agents and Chemotherapy·Gaelle CuzonThierry Naas
Jan 6, 2017·Antimicrobial Agents and Chemotherapy·Rémy A BonninThierry Naas
Oct 26, 2018·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·Hyukmin LeeKwang Jun Lee

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
PCRs

Software Mentioned

Kor

Related Concepts

Related Feeds

Carbapenems

Carbapenems are members of the beta lactam class of antibiotics and are used for the treatment of severe or high-risk bacterial infections. Discover the latest research on carbapenems here.

Carbapenems (ASM)

Carbapenems are members of the beta lactam class of antibiotics and are used for the treatment of severe or high-risk bacterial infections. Discover the latest research on carbapenems here.