Development of a UHPLC-MS/MS method for the determination of 84 pharmaceuticals and drugs of abuse in human liver

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Amvrosios OrfanidisN Raikos

Abstract

Analysis of post-mortem liver for toxicological reasons is a considerable option when blood is unavailable. The development of analytical methods for tissue specimens is not as straightforward as for biological fluids as tissue presents challenges to the analytical chemist. The present study reports the development of a UHPLC-MS/MS method for the detection and quantification of 84 drugs and pharmaceuticals in human liver. The selected target drugs include pharmaceutical drugs and drugs of abuse. Sample preparation was studied using QuEChERS and different ratios of solvent volume and sample mass. Best results were attained by homogenizing 1 g of liver with acetonitrile K2CO3 buffer (pH = 10), QuEChERS salts MgSO4/ NaCl (1st purification step) and PSA/ 150 mg MgSO4 (2nd purification step). The extracted sample was analysed on UHPLC-MS/MS in multiple reaction monitoring mode (MRM) on a reversed-phase (Acquity BEH C18) column. Elution was accomplished by gradient program of mobile phase A: water, 0.1% formic acid and B: methanol, 0.1% formic acid that lasted 17 min. The method was specific, without interferences from the complex matrix. Sensitivity was satisfactory with limit of detection (LOD) ranging from 0.01 ng/g to 4.94 ng/g. ...Continue Reading

Citations

Dec 15, 2020·Critical Reviews in Analytical Chemistry·Janvi Shah, Rajendra Kotadiya
Jan 14, 2022·Journal of Analytical Toxicology·Gabriela de Paula MeirellesMauricio Yonamine

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