Development of a versatile two-photon endoscope for biological imaging

Biomedical Optics Express
Youbo ZhaoRobert J Gordon

Abstract

We describe a versatile, catheter-type two-photon probe, designed for in vivo and ex vivo imaging of the aqueous outflow pathway in the eye. The device consists of a silica double cladding fiber used for laser delivery and fluorescence collection, a spiral fiber scanner driven by a miniature piezoelectric tube, and an assembly of three micro-size doublet achromatic lenses used for focusing the laser and collecting the two-photon excitation signal. All the components have a maximum diameter of 2 mm and are enclosed in a length of 12-gauge stainless steel hypodermic tubing having an outer diameter of 2.8 mm. The lateral and axial resolutions of the probe are measured to be 1.5 μm and 9.2 μm, respectively. Different lens configurations and fibers are evaluated by comparing their spatial resolutions and fluorescence signal collection efficiencies. Doublet achromatic lenses and a double cladding fiber with a high inner cladding numerical aperture are found to produce a high signal collection efficiency, which is essential for imaging live tissues. Simple methods for reducing image distortions are demonstrated. Images of human trabecular meshwork tissue are successfully obtained with this miniature two-photon microscope.

References

Apr 6, 1990·Science·W DenkW W Webb
Mar 1, 1996·Microscopy Research and Technique·H GongB J Tripathi
May 16, 2003·Journal of the Optical Society of America. A, Optics, Image Science, and Vision·Min Gu, Damian Bird
Jun 21, 2003·Optics Letters·Juergen C Jung, Mark J Schnitzer
Jul 30, 2003·Optics Letters·M T MyaingP St J Russell
Nov 5, 2003·Nature Biotechnology·Warren R ZipfelWatt W Webb
May 7, 2004·Journal of Neurophysiology·Juergen C JungMark J Schnitzer
Sep 1, 2005·Experimental Eye Research·Sami ToyranDeepak P Edward
Sep 30, 2005·Optics Letters·Benjamin A FlusbergMark J Schnitzer
Nov 22, 2005·Nature Methods·Benjamin A FlusbergMark J Schnitzer
Jan 3, 2006·Experimental Eye Research·Mark Johnson
Feb 16, 2006·Quarterly Reviews of Biophysics·Alberto DiasproMaddalena Collini
Apr 22, 2006·Optics Letters·Mon Thiri MyaingXingde Li
Mar 30, 2007·Microscopy Research and Technique·K KönigM Kaatz
May 31, 2007·Journal of Microscopy·L Fu, M Gu
Feb 1, 1983·Applied Optics·D C Leiner, R Prescott
Apr 15, 1985·Applied Optics·K TanakaK Hauchi
Mar 5, 2008·Journal of Biomedical Optics·Pilhan KimSeok H Yun
Jun 11, 2008·Optics Express·Christoph J EngelbrechtFritjof Helmchen
Aug 20, 2008·Optics Express·Yu-Chung ChangTheodore B Norris
Oct 7, 2008·Investigative Ophthalmology & Visual Science·Hiroshi NakamuraDeepak P Edward
Feb 26, 2009·Experimental Eye Research·Ernst R Tamm
Mar 5, 2009·Journal of Biomedical Optics·Peng XiMarcos Dantus
Jun 23, 2009·Applied Optics·R Le HarzicB Messerschmidt
Apr 1, 1980·Applied Optics·K Nishizawa
Jul 1, 2010·Optics Express·Simon Lemire-RenaudCaroline Boudoux

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Citations

Feb 12, 2014·Optics Express·Hsiang-Yu ChungChi-Kuang Sun
Dec 18, 2015·Scientific Reports·Guillaume DucourthialFrédéric Louradour
May 9, 2012·Journal of Biomedical Optics·Christopher M BrownChris Xu
Sep 4, 2015·The Review of Scientific Instruments·Joonkyu ParkPaul G Evans
Feb 16, 2013·Biomedical Optics Express·Matthew Kyrish, Tomasz S Tkaczyk
Apr 20, 2011·Optics Express·Matthew KyrishTomasz S Tkaczyk
Jan 13, 2012·Current Opinion in Neurobiology·Jason N D Kerr, Axel Nimmerjahn
Dec 24, 2018·Journal of Biomedical Optics·Lin HuangShuo Tang
Jan 1, 2017·Light, Science & Applications·Wenxuan LiangXingde Li
Jan 10, 2012·Annals of Biomedical Engineering·Athanasios SarantopoulosVasilis Ntziachristos
Aug 15, 2014·Journal of Biomedical Optics·Aleksandra Klimas, Emilia Entcheva
May 16, 2018·Biomedical Optics Express·Farhad AkhoundiKhanh Kieu
Nov 16, 2021·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Vytautas KučikasMarc A M J van Zandvoort

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Methods Mentioned

BETA
fluorescence collection

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