Development of a whole-cell biocatalyst co-expressing P450 monooxygenase and glucose dehydrogenase for synthesis of epoxyhexane

Applied Microbiology and Biotechnology
Akasit SiriphongphaewThunyarat Pongtharangkul

Abstract

Oxygenases-based Escherichia coli whole-cell biocatalyst can be applied for catalysis of various commercially interesting reactions that are difficult to achieve with traditional chemical catalysts. However, substrates and products of interest are often toxic to E. coli, causing a disruption of cell membrane. Therefore, organic solvent-tolerant bacteria became an important tool for heterologous expression of such oxygenases. In this study, the organic solvent-tolerant Bacillus subtilis 3C5N was developed as a whole-cell biocatalyst for epoxidation of a toxic terminal alkene, 1-hexene. Comparing to other hosts tested, high level of tolerance towards 1-hexene and a moderately hydrophobic cell surface of B. subtilis 3C5N were suggested to contribute to its higher 1,2-epoxyhexane production. A systematic optimization of reaction conditions such as biocatalyst and substrate concentration resulted in a 3.3-fold increase in the specific rate. Co-expression of glucose dehydrogenase could partly restored NADPH-regenerating ability of the biocatalyst (up to 38 % of the wild type), resulting in approximately 53 % increase in specific rate representing approximately 22-fold increase in product concentration comparing to that obtained prior...Continue Reading

References

Jan 1, 1975·Methods in Enzymology·G Avigad, S Englard
Apr 1, 1993·Applied and Environmental Microbiology·M I Voskuil, G H Chambliss
Apr 1, 1997·Biochimica Et Biophysica Acta·T GitlesenP Adlercreutz
Jan 22, 1998·Applied and Environmental Microbiology·M J HuertasJ L Ramos
Nov 28, 2001·Applied and Environmental Microbiology·Q S LiS Shimizu
Oct 9, 2002·Nature Biotechnology·Anton GliederFrances H Arnold
Sep 6, 2003·Applied and Environmental Microbiology·María-Isabel Ramos-GonzálezJuan L Ramos
Oct 22, 2005·Chemistry : a European Journal·Takafumi KuboFrances H Arnold
Dec 8, 2005·Applied and Environmental Microbiology·Nick J P WierckxJan Wery
Jun 13, 2006·Biotechnology and Bioengineering·Jin-Byung ParkAndreas Schmid
Jul 25, 2006·Biotechnology and Bioengineering·Christopher V F Baldwin, John M Woodley
Nov 13, 2008·Journal of Bioscience and Bioengineering·Takahiro HamadaHisao Ohtake
Jan 17, 2009·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·G ThedeiP Ciancaglini
May 12, 2011·Journal of Industrial Microbiology & Biotechnology·Sjef CornelissenBruno Bühler

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Citations

May 23, 2014·Applied Microbiology and Biotechnology·Rita Bernhardt, Vlada B Urlacher
Apr 4, 2015·Current Opinion in Biotechnology·Christian WillrodtMattijs K Julsing
Aug 15, 2013·Microbial Biotechnology·Daniel SchepsBernhard Hauer
Feb 6, 2015·Applied Microbiology and Biotechnology·Marie T Lundemo, John M Woodley
Dec 17, 2014·Applied Microbiology and Biotechnology·Harini VenkataramanLubbert Dijkhuizen
Apr 9, 2014·Current Opinion in Plant Biology·Hugues RenaultDanièle Werck-Reichhart
Oct 25, 2014·Bioprocess and Biosystems Engineering·Somwit ArchakunakornJirarut Wongkongkatep
May 18, 2016·Journal of Biotechnology·Marko KirtzBernhard Hauer
Oct 25, 2016·Journal of Industrial Microbiology & Biotechnology·C A MüllerUlrich Schwaneberg
May 24, 2014·Dalton Transactions : an International Journal of Inorganic Chemistry·Armin H MeyerMartin Elsner
Jun 7, 2014·Chemical Communications : Chem Comm·Dirk HoltmannFrank Hollmann
Apr 17, 2013·Biotechnology and Applied Biochemistry·Stephen Graham BellLuet-Lok Wong
Sep 15, 2016·Neural Computation·Sou Nobukawa, Haruhiko Nishimura

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