PMID: 15352485Sep 9, 2004Paper

Development of a whole-sediment toxicity test using a benthic marine microalga

Environmental Toxicology and Chemistry
M S Adams, Jennifer L Stauber

Abstract

An acute whole-sediment toxicity test with a benthic marine microalga was developed and optimized using flow cytometry to distinguish algae (based on their chlorophyll a autofluorescence) from sediment particles. Of seven benthic marine algae screened, the diatom Entomoneis cf punctulata was most suitable because of its tolerance of a wide range of water and sediment physicochemical parameters, including salinity, pH, ammonia, and sulfide. A whole-sediment and water-only toxicity test based on inhibition of esterase activity in this species was developed. Enzyme activity rather than growth was used as the test endpoint, as nutrient release from sediments has previously been found to stimulate algal growth, potentially masking contaminant toxicity. The sensitivity of the bioassay to a range of metals (copper, zinc, cadmium, lead, arsenic, manganese) and phenol in water-only exposures was compared to the standard 72-h growth rate inhibition test. The esterase enzyme inhibition test was sensitive to copper, with a 3-h inhibitory concentration to cause a 50% (IC50) reduction in a fluorescein diacetate fluorescence value of 97 +/- 39 microg Cu/L. A concentration-dependent response was also observed in the presence of sediment partic...Continue Reading

Citations

Jun 16, 2007·Archives of Environmental Contamination and Toxicology·D SchlenkJ T Oris
May 2, 2008·Archives of Environmental Contamination and Toxicology·D StromJ L Stauber
Feb 10, 2011·Archives of Environmental Contamination and Toxicology·Daniel J WardDianne F Jolley
Aug 27, 2009·Environmental Technology·L VolatierY Perrodin
Mar 26, 2013·Ecotoxicology and Environmental Safety·Liping LouYingxu Chen
Sep 23, 2011·Chemosphere·Stuart L SimpsonDavid A Spadaro
May 25, 2010·Environment International·Cristiano V M AraújoIgnacio Moreno-Garrido
Dec 25, 2009·Ecotoxicology and Environmental Safety·A MauffretJ Blasco
Nov 13, 2009·The Science of the Total Environment·Cristiano V M AraújoIgnacio Moreno-Garrido
May 12, 2009·Ecotoxicology and Environmental Safety·Bibiana DebeliusLuis M Lubián
Dec 13, 2006·Environment International·I Moreno-GarridoJ Blasco
Jun 3, 2006·Marine Environmental Research·M T Binet, J L Stauber
Dec 22, 2005·Ecotoxicology and Environmental Safety·Yang YuXiaoli Shi
Feb 24, 2015·Environmental Toxicology and Chemistry·Francesca GissiDianne F Jolley
Nov 30, 2006·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Rossella GrandeLuigina Cellini
Nov 10, 2017·Frontiers in Microbiology·Alice RotiniLuciana Migliore
Jul 22, 2008·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Hongling LiuXiaorong Wang
Jul 15, 2006·Environmental Toxicology·P M DellamatriceS Alleau
Feb 28, 2021·Journal of Environmental Sciences (China)·Xin MengBaoqing Shan

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