Development of acid stable, hyper-crosslinked, silica-based reversed-phase liquid chromatography supports for the separation of organic bases

Journal of Chromatography. a
Lianjia MaPeter W Carr

Abstract

A new generation of extremely acid stable "hyper-crosslinked" (HC) phases have been developed with good plate counts for basic drug separations. In our previous work, we successfully developed an approach for synthesizing HC stationary phases on silica substrates using aluminum trichloride catalyzed Friedel-Crafts (F-C) chemistry to improve the stability of silica-based RPLC stationary phases at low pH. However, the performance of basic analytes on these HC phases under acidic conditions was unusually poor compared to that of conventional silica-based C18 phases. The effects of the specific F-C catalysts used and the specific silica substrate on the chromatographic properties of HC phases have been studied. Modified synthetic strategies that give both good observed plate counts for basic analytes under acidic conditions and very good low pH stability without compromising other chromatographic properties of the hyper-crosslinked phases have been developed. Replacement of aluminum trichloride with tin tetrachloride as the catalyst for the F-C chemistry and use of a very high purity silica result in significantly improved plate counts for basic analytes. In formic acid buffered mobile phases, which are highly compatible with elect...Continue Reading

Citations

Feb 16, 2008·Analytical and Bioanalytical Chemistry·H Luo, P W Carr
Apr 8, 2017·Journal of Chromatographic Science·Rui WangXingzhong Guo
Feb 4, 2019·Journal of Chromatography. a·Erin P Shields, Stephen G Weber
Apr 15, 2019·Journal of Chromatography. a·Erin P Shields, Stephen G Weber

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