Development of amino acid derivatization reagents for liquid chromatography electrospray ionization mass spectrometric analysis and ionization efficiency measurements

Journal of Chromatography. a
Riin RebaneKoit Herodes

Abstract

Derivatization is one of the most common ways for improving chromatographic separation and sensitivity for LC-ESI-MS analysis. The aim of this work was to design new derivatization reagents for LC-ESI-MS analysis of amino acids which would (1) provide good reversed phase chromatographic separation, (2) most importantly, provide low detection limits, (3) be easily synthesized, (4) produce derivatives which are less susceptible to matrix influences and (5) have convenient derivatization procedure with stable derivatives suitable for automatization. In the current work two new LC-ESI-MS compatible derivatization reagents have been designed and synthesized, dibenzyl ethoxymethylene malonate (DBEMM) and benzyl ethyl ethoxymethylene malonate (EBEMM). The DBEMM meets all the goals set with instrumental detection limits as low as 1 femtomole for amino acids and 40 attomole for selenoamino acids.

References

Jun 22, 2006·Current Drug Metabolism·Mohammed Jemal, Yuan-Qing Xia
Feb 1, 2007·Journal of Agricultural and Food Chemistry·Sergio Gómez-AlonsoEsteban García-Romero
Jan 10, 2008·Rapid Communications in Mass Spectrometry : RCM·Ivo LeitoRisto Tanner
Feb 29, 2008·Journal of Chromatography. a·Anneli KruveIvo Leito
Mar 12, 2010·Analytical Chemistry·Merit OssIvo Leito
Mar 23, 2010·Journal of Chromatography. a·Michal HolcapekMiroslav Lísa
Aug 15, 2012·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Riin RebaneKoit Herodes
Jun 1, 2013·Journal of Mass Spectrometry : JMS·Anneli KruveIvo Leito
Mar 19, 2014·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Riin RebaneKoit Herodes

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Citations

Oct 14, 2017·European Journal of Mass Spectrometry·Maarja-Liisa OldekopKoit Herodes
Dec 24, 2018·Journal of Separation Science·James AsensoDao-Quan Tang
Aug 6, 2019·Rapid Communications in Mass Spectrometry : RCM·Riin RebaneIvo Leito
Oct 20, 2015·Bioanalysis·Yunting ZhuDafang Zhong
Jul 11, 2019·European Journal of Mass Spectrometry·Riin Rebane, Koit Herodes
Oct 22, 2020·Biomedical Chromatography : BMC·Victor DavidToma Galaon

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