Development of an alcohol-inducible gene expression system for recombinant protein expression in Chlamydomonas reinhardtii

Journal of Applied Phycology
Sujin LeeHee-Sik Kim

Abstract

Microalgae have been widely considered for the production of valuable products, such as lipid-based biofuel, value-added pigments, and anti-photo aging reagents. More recently, microalgae have been considered an alternative host for recombinant protein production because of their economic benefits and ecofriendly characteristics. Additionally, many microalgal strains identified to date are generally recognized as safe (GRAS); therefore, the use of microalgae-based technology is promising. However, basic studies on the genetic engineering of microalgae are rare, despite their importance. Particularly, inducible promoter systems that can be applied for strain engineering or recombinant protein production are rarely studied; hence, a number of challenging issues remain unsolved. Therefore, in this study, we focused on the development of a convenient and compact-inducible promoter system that can be used in microalgae. Based on previous success with plant systems, we employed the alcohol-inducible AlcR-P alcA system, which originates from the filamentous fungus, Aspergillus nidulans. This system comprises only two components, a regulatory protein, AlcR, and an inducible promoter, P alcA. Therefore, construction and transformation o...Continue Reading

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Citations

Mar 7, 2020·Expert Opinion on Biological Therapy·Sergio Rosales-MendozaBernardo Bañuelos-Hernandez
Jun 23, 2019·Applied Microbiology and Biotechnology·Yan-Zhen MeiChuan-Chao Dai
Nov 24, 2020·Biochimie·Meng-Ping ZhangChuan Wang
Oct 31, 2020·Frontiers in Bioengineering and Biotechnology·Myra N ChávezJörg Nickelsen
Jan 12, 2022·ACS Synthetic Biology·Tessema K KassawGraham Peers

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Methods Mentioned

BETA
glycosylation
PCR
transgenic

Software Mentioned

ImageJ

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