Development of an amperometric-based glucose biosensor to measure the glucose content of fruit

PloS One
Lee Fung AngMun Fei Yam

Abstract

An amperometric enzyme-electrode was introduced where glucose oxidase (GOD) was immobilized on chitosan membrane via crosslinking, and then fastened on a platinum working electrode. The immobilized enzyme showed relatively high retention activity. The activity of the immobilized enzyme was influenced by its loading, being suppressed when more than 0.6 mg enzyme was used in the immobilization. The biosensor showing the highest response to glucose utilized 0.21 ml/cm2 thick chitosan membrane. The optimum experimental conditions for the biosensors in analysing glucose dissolved in 0.1 M phosphate buffer (pH 6.0) were found to be 35°C and 0.6 V applied potential. The introduced biosensor reached a steady-state current at 60 s. The apparent Michaelis-Menten constant ([Formula: see text]) of the biosensor was 14.2350 mM, and its detection limit was 0.05 mM at s/n > 3, determined experimentally. The RSD of repeatability and reproducibility of the biosensor were 2.30% and 3.70%, respectively. The biosensor was showed good stability; it retained ~36% of initial activity after two months of investigation. The performance of the biosensors was evaluated by determining the glucose content in fruit homogenates. Their accuracy was compared t...Continue Reading

References

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Citations

Dec 17, 2016·Membranes·Tanmay Kulkarni, Gymama Slaughter
Mar 4, 2017·Materials Science & Engineering. C, Materials for Biological Applications·Vuslat BukKaan Cebesoy Emregul
Jun 15, 2018·Sensors·Melis Asalİlker Polatoğlu
Dec 5, 2019·Polymers·Dipali R Bagal-Kestwal, Been-Huang Chiang
Sep 15, 2018·Scientific Reports·Nur Syafinaz RidhuanZainovia Lockman
Nov 1, 2016·Comprehensive Reviews in Food Science and Food Safety·Weihong HuTingtiao Pan
Apr 13, 2018·Analytical Biochemistry·Fausto N CombaAna M Baruzzi

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Methods Mentioned

BETA
biosensor
biosensors
biosensing

Software Mentioned

GraphPad

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