Development of an Analytical Method for Analyzing Pyrrolizidine Alkaloids in Different Groups of Food by UPLC-MS/MS

Journal of Agricultural and Food Chemistry
Stephen W C Chung, Chi-Ho Lam

Abstract

Suspected nontargeted pyrrolizidine alkaloids (PAs), without analytical reference standard, were observed and interfered with the determination of targeted PAs in complex food matrices, especially for spices samples. Selectivity and applicability of multiple reaction monitoring (MRM) transitions, multistage fragmentation (MS3), and MRM with differential ion mobility spectrometry (DMS) for eliminating false positive identifications were evaluated. Afterward, a selective and sensitive LC-MS/MS method for the determination of 15 PAs and 13 PA N-oxides in foodstuffs was developed. The sample preparation and cleanup are applicable to a wide range of foodstuffs, including cereal products, dairy products, meat, eggs, honey, tea infusion, and spices. Freezing-out of the raw extract and the water/acetonitrile washing steps in a solid phase extraction was found to efficiently remove complex matrices. The method was validated at 0.05 μg kg-1 for general food and 0.5 μg kg-1 for spices, with reference to the Eurachem Guide. The estimated limit of quantifications of different PAs was in the range of 0.010-0.087 μg kg-1 for general food and 0.04-0.76 μg kg-1 for spices. Isotopically labeled PAs were used as internal standards to correct the ...Continue Reading

References

Mar 2, 2011·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·L A P HoogenboomH P Van Egmond
Aug 9, 2011·Journal of Chromatography. a·Carmen FerrerA R Fernández-Alba
Feb 12, 2014·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Lukas VaclavikJeanne I Rader
Oct 21, 2015·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Elizabeth M MudgePaula N Brown
Oct 21, 2016·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Patrick P J MulderRon L A P Hoogenboom

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Citations

Sep 13, 2019·Analytical and Bioanalytical Chemistry·Florian KaltnerChristoph Gottschalk
Aug 14, 2019·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Zhimin LuoQiang Fu
Mar 22, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Li-Hua ChenJian-Yong Si
Nov 21, 2020·Food Chemistry·Patricia BrugnerottoAna Carolina Oliveira Costa
Jan 11, 2021·Journal of Pharmaceutical and Biomedical Analysis·Keabetswe MasikeAndré de Villiers

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