Development of an Antigen Delivery Platform Using Lactobacillus acidophilus Decorated With Heterologous Proteins: A Sheep in Wolf's Clothing Story

Frontiers in Microbiology
Paula J UrizaGabriel Briones

Abstract

S-layers are bacterial structures present on the surface of several Gram-positive and Gram-negative bacteria that play a role in bacterial protection. In Lactobacillus acidophilus (L. acidophilus ATCC 4356), the S-layer is mainly composed of the protein SlpA. A tandem of two copies of the protein domain SLP-A (pfam: 03217) was identified at the C-terminal of SlpA, being this double SLP-A protein domain (in short dSLP-A) necessary and sufficient for the association of the protein to the L. acidophilus cell wall. A variety of proteins fused to the dSLP-A domain were able to spontaneously associate with high affinity to the cell wall of L. acidophilus and Bacillus subtilis var. natto, in a process that we termed decoration. Binding of dSLP-A-containing-proteins to L. acidophilus was stable at conditions that mimic the gastrointestinal transit in terms of pH, proteases, and bile salts. To evaluate if protein decoration of L. acidophilus can be adapted to generate an oral vaccine platform, a chimeric antigen derived from the bacterial pathogen Shiga-toxin-producing Escherichia coli (STEC) was constructed by fusing the sequences encoding the polypeptides EspA36-192, Intimin653-953, Tir240-378, and H7 flagellin352-374 (EITH7) to the d...Continue Reading

References

Feb 1, 1997·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·R GallerG F Mann
Jun 7, 2005·FEMS Microbiology Reviews·Silja Avall-Jääskeläinen, Airi Palva
Aug 7, 2007·Expert Review of Anti-infective Therapy·Paul N Goldwater
Sep 26, 2007·Glycobiology·María Virginia TribulattiOscar Campetella
Dec 3, 2008·Proceedings of the National Academy of Sciences of the United States of America·Sergey R KonstantinovYvette van Kooyk
Jul 19, 2011·Journal of Bioscience and Bioengineering·Nobuhisa AshidaNaoyuki Yamamoto
May 19, 2012·Biochemical and Biophysical Research Communications·María Guadalupe MartínezSandra M Ruzal
Jul 26, 2012·Nature Reviews. Immunology·Nils Lycke
Feb 4, 2014·FEMS Microbiology Reviews·Uwe B SleytrDietmar Pum
Aug 20, 2014·Proceedings of the National Academy of Sciences of the United States of America·Stanley Plotkin
Oct 13, 2015·Journal of Controlled Release : Official Journal of the Controlled Release Society·Andrés Esteban IbañezJuliana Cassataro
Oct 18, 2015·Applied and Environmental Microbiology·Brant R JohnsonTodd R Klaenhammer
Feb 28, 2016·Applied and Environmental Microbiology·Jeffrey P HymesTodd R Klaenhammer
Jul 5, 2016·Applied Microbiology and Biotechnology·María Mercedes PalominoSandra M Ruzal
Mar 16, 2017·Journal of Applied Microbiology·K SzatrajM Chmielewska-Jeznach
Apr 12, 2017·Proceedings of the National Academy of Sciences of the United States of America·Walter A Orenstein, Rafi Ahmed
Mar 17, 2019·Biotechnology Advances·François P Douillard, Willem M de Vos
May 6, 2019·Applied Microbiology and Biotechnology·Joaquina Fina MartinSandra M Ruzal

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Methods Mentioned

BETA
PCR
electrophoresis
ELISA
affinity purification
confocal microscopy

Software Mentioned

BLAST
Image Studio
SignalP
GraphPad

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