Development of an assay for a biomarker of pregnancy and early fetal loss

Environmental Health Perspectives
R E CanfieldA J Wilcox

Abstract

Human chorionic gonadotropin (hCG) is a glycoprotein hormone, secreted by the syncytiotrophoblast cells of the fertilized ovum, that enters the maternal circulation at the time of endometrial implantation. It is composed of two nonidentical subunits; alpha and beta, with molecular weights of 14 kD and 23 kD, respectively. Its alpha subunit is identical in primary structure to its glycoprotein homologs, luteinizing hormone (LH), follicle-stimulating hormone (FSH), and thyroid-stimulating hormone (TSH). Human chorionic gonadotropin binds to the same receptor as hLH and displays the same biological response, namely, to stimulate the declining function of the corpus luteum to produce progestins and estrogen late in the menstrual cycle. The differences in the structures of hCG and hLH have been exploited to develop antibodies that can measure hCG specifically in the presence of hLH. Two-site antibody binding assays have been developed, based on a surface immunological concept of hCG epitopes, that involve four distinct regions to which antibodies against hCG can bind simultaneously. Antibody cooperative effects, in conjunction with kinetic advantages derived from the concentration factors by use of the sandwich assay technique (immu...Continue Reading

References

Aug 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·H C ChenG T Ross
Jun 20, 1978·Annals of the New York Academy of Sciences·G M Fuller
Jan 1, 1978·The Journal of Physiology·D GarnierO Rougier
Nov 1, 1978·Acta Endocrinologica·D M RobertsonE Diczfalusy
Jun 1, 1985·American Journal of Reproductive Immunology and Microbiology : AJRIM·P H EhrlichR E Canfield
Jan 16, 1985·Biochemical and Biophysical Research Communications·L A ColeF Perini
Sep 20, 1986·British Medical Journal·R R Gordon
Nov 1, 1987·Environmental Health Perspectives·A J WilcoxR E Canfield
May 1, 1986·American Journal of Public Health·M L Doshi
Jul 15, 1972·American Journal of Obstetrics and Gynecology·J L VaitukaitisG T Ross
Nov 15, 1973·Molecular and Cellular Biochemistry·F J MorganR E Canfield
Jul 25, 1972·Biochemical and Biophysical Research Communications·O P BahlN Swaminathan
Jul 27, 1970·Biochemical and Biophysical Research Communications·N Swaminathan, O P Bahl
Jan 1, 1969·Journal of Clinical Pathology·J L Bell
Sep 13, 1980·Lancet·J F MillerA Sykes
May 21, 1983·Lancet·P G WhittakerT Lind
Jan 1, 1981·Annual Review of Biochemistry·J G Pierce, T F Parsons
Aug 27, 1981·The New England Journal of Medicine
Dec 1, 1982·The Journal of Clinical Endocrinology and Metabolism·H Suginami, A Kawaoi
Dec 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·T F Parsons, J G Pierce
Oct 1, 1982·Fertility and Sterility·D K EdmondsP J Wood
Oct 1, 1960·Acta Endocrinologica·L WIDE, C A GEMZELL

❮ Previous
Next ❯

Citations

Oct 1, 1987·Environmental Health Perspectives·Z Stein, M Hatch
Jun 1, 1995·Journal of Epidemiology and Community Health·M JoffeM Vessey
Jul 1, 1993·Environmental Health Perspectives·J F Cordero
May 1, 1997·Occupational and Environmental Medicine·M Joffe
Mar 13, 2009·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Sarit YanivDavid Elad
May 20, 2016·Critical Reviews in Clinical Laboratory Sciences·Hui YaoHaibo Li
Jun 12, 2012·Biochimica Et Biophysica Acta·Y E M KootN S Macklon
Oct 1, 1987·Environmental Health Perspectives·R K Miller
Jan 1, 1992·British Journal of Industrial Medicine·M Joffe
Jul 26, 2011·Human Reproduction·Yvonne E M KootNick S Macklon
May 28, 2010·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Anne Marie Z JukicDonna D Baird
Jun 6, 2020·The Analyst·Dimple ChavanRichard C Willson
Aug 1, 1991·Environmental Health Perspectives·S H Swan, B L Lasley
Jan 1, 1991·Environmental Health Perspectives·M C Hatch, G Friedman-Jimenez
Jan 27, 2021·Analytical Chemistry·Sara Tortorella, Stefano Cinti
Jul 10, 2021·Molecular Autism·A TsompanidisS Baron-Cohen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.