Development of an assay of plasma neurofilament light chain utilizing immunomagnetic reduction technology

PloS One
Huei-Chun LiuShieh-Yueh Yang

Abstract

Axonal damage leads to the release of neurofilament light chain (NFL), which enters the CSF or blood. In this work, an assay kit for plasma NFL utilizing immunomagnetic reduction (IMR) was developed. Antibodies against NFL were immobilized on magnetic nanoparticles to develop an IMR NFL kit. The preclinical properties, such as the standard curve, limit of detection (LoD), and dynamic range, were characterized. Thirty-one normal controls (NC), fifty-two patients with Parkinson's disease (PD) or PD dementia (PDD) and thirty-one patients with Alzheimer's disease (AD) were enrolled in the study evaluating the plasma NFL assay using an IMR kit. T-tests and receiver operating characteristic (ROC) curve analysis were performed to investigate the capability for discrimination among the clinical groups according to plasma NFL levels. The LoD of the NFL assay using the IMR kit was found to be 0.18 fg/ml. The dynamic range of the NFL assay reached 1000 pg/ml. The NC group showed a plasma NFL level of 7.70 ± 4.00 pg/ml, which is significantly lower than that of the PD/PDD (15.85 ± 7.82 pg/ml, p < 0.001) and AD (19.24 ± 8.99 pg/ml, p < 0.001) groups. A significant difference in plasma NFL levels was determined between the PD and AD groups (...Continue Reading

References

Mar 1, 1992·Journal of Neurology, Neurosurgery, and Psychiatry·A J HughesA J Lees
Dec 29, 2004·Lancet Neurology·Charlotte E TeunissenChris Polman
Feb 1, 2005·Journal of Immunological Methods·W J A Van GeelM M Verbeek
Feb 23, 2007·Journal of Neurology, Neurosurgery, and Psychiatry·D de JongM M Verbeek
Jun 18, 2010·Journal of Alzheimer's Disease : JAD·Jeroen J J van EijkMarcel M Verbeek
Nov 28, 2012·Multiple Sclerosis : Clinical and Laboratory Research·Jeppe Romme ChristensenFinn Sellebjerg
Apr 5, 2013·Alzheimer's Research & Therapy·Henrik ZetterbergOskar Hansson
May 31, 2013·BMC Neurology·Maria Landqvist WaldöElisabet Englund
Nov 3, 2015·JAMA Neurology·Henrik ZetterbergUNKNOWN Alzheimer’s Disease Neuroimaging Initiative
Dec 20, 2015·Neurochemistry International·Edyta MerissonJonas Åkeson
Sep 9, 2016·Annals of Clinical and Translational Neurology·Lieke H MeeterJohn C van Swieten
Nov 8, 2016·Scientific Reports·Pashtun ShahimKaj Blennow
Feb 10, 2017·Neurology·Oskar HanssonUNKNOWN Swedish BioFINDER study
Mar 28, 2017·JAMA Neurology·Niklas MattssonUNKNOWN Alzheimer’s Disease Neuroimaging Initiative
Jan 26, 2018·Alzheimer's Research & Therapy·Samir Abu-RumeilehPiero Parchi
Jul 30, 2018·Alzheimer's Research & Therapy·Piotr LewczukHenrik Zetterberg
Sep 2, 2018·Nature Reviews. Neurology·Michael KhalilJens Kuhle
Feb 9, 2019·Movement Disorders : Official Journal of the Movement Disorder Society·David José Dávila-Ortiz de MontellanoJulio Sotelo
May 28, 2019·Journal of Neurology, Neurosurgery, and Psychiatry·Lieke H H MeeterJohn C Van Swieten

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Methods Mentioned

BETA
blood draw
enzyme-linked immunosorbent assay
ELISA

Software Mentioned

GraphPad Prism
MedCalc

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