Development of an attenuated strain of chikungunya virus for use in vaccine production

Vaccine
N H LevittH W Lupton

Abstract

An attenuated chikungunya (CHIK) virus clone was developed for production of a live vaccine for human use. CHIK strain 15561 was subjected to 18 plaque-to-plaque passages in MRC-5 cultures before CHIK 181/clone 25 was selected as vaccine seed based on homogeneous small plaque size, suckling mouse avirulence, reduced monkey viraemia and genetic stability. Oligonucleotide mapping demonstrated differences between parent and clone. Vaccine (pilot-lot production) elicited neutralizing antibody and protected mice and rhesus monkeys against challenge. After challenge, viraemias were absent in vaccinated monkeys. Vaccine was then produced and tested in accordance with governmental regulatory requirements of human use.

References

Jan 1, 1957·Transactions of the New York Academy of Sciences·J CASALS

Citations

Feb 12, 2009·Journal of Biosciences·A B Sudeep, D Parashar
Jul 3, 2013·Antiviral Research·Simon-Djamel ThibervilleXavier de Lamballerie
Oct 2, 2013·Journal of Medicinal Chemistry·Adel A RashadPaul A Keller
Jun 17, 2010·Nature Reviews. Microbiology·Olivier Schwartz, Matthew L Albert
May 3, 2013·Journal of Virology·Claudia Sánchez-San MartínMargaret Kielian
Feb 25, 2010·The Journal of Clinical Investigation·Karine LabadiePierre Roques
May 4, 2010·PLoS Neglected Tropical Diseases·Michelle M ThiboutotKaruppiah Muthumani
Mar 22, 2013·PLoS Neglected Tropical Diseases·Stefan W MetzGorben P Pijlman
Jul 25, 2014·PLoS Neglected Tropical Diseases·James Weger-LucarelliJorge E Osorio
Jul 8, 2011·The American Journal of Tropical Medicine and Hygiene·Laura J SutherlandA Desiree LaBeaud
Jun 3, 2011·The American Journal of Tropical Medicine and Hygiene·Justin R DarwinScott C Weaver
Jun 24, 2014·BioMed Research International·Deepti Parashar, Sarah Cherian
Aug 10, 2004·Journal of Occupational and Environmental Medicine·Janice M RusnakPhillip Pittman
Sep 22, 2015·Pathogens·Rebecca M BroeckelDaniel N Streblow
Feb 9, 2016·Virology·Rachmat HidajatPeter Pushko
Aug 12, 2008·Vaccine·Eryu WangScott C Weaver
Apr 24, 2007·The Lancet Infectious Diseases·G PialouxM Strobel
Dec 25, 2010·International Journal of Dermatology·Jaheersha PakranLekha Thumbayil
Apr 22, 2015·Vector Borne and Zoonotic Diseases·Tero AholaPeter Liljeström
May 27, 2006·Médecine et maladies infectieuses·G PialouxM Strobel
Nov 16, 2012·Expert Review of Vaccines·Scott C WeaverDan T Stinchcomb
Jul 24, 2014·The Journal of General Virology·Wadie D Mahauad-FernandezChioma M Okeoma
Jun 14, 2015·Antiviral Research·Edward McSweeganSharon Hrynkow
Jun 16, 2015·Vaccine·Jorge A Salazar-GonzálezSergio Rosales-Mendoza
Jul 22, 2011·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Rojjanaporn PulmanausahakulDuncan R Smith
Apr 7, 2015·The Journal of Allergy and Clinical Immunology·Caroline PetitdemangeVincent Vieillard
Sep 4, 2012·Vaccine·Charles H HokeDavid W Vaughn
Sep 8, 2009·Virology·Maxime SolignatChristian Devaux
Sep 15, 2015·Current Tropical Medicine Reports·Kristin M Long, Mark T Heise
Nov 12, 2015·Human Vaccines & Immunotherapeutics·Michael SchwameisBernd Jilma
Sep 5, 2015·PLoS Neglected Tropical Diseases·Kenneth PlanteScott C Weaver
Jul 28, 2016·Cell Reports·David W HawmanThomas E Morrison
Sep 4, 2016·Virology Journal·Pattra ChalaemKamoltip Promnares
Sep 8, 2010·Expert Review of Anti-infective Therapy·Marie Christine Jaffar-BandjeePhilippe Gasque
Dec 20, 2016·Nature Medicine·Jesse H ErasmusScott C Weaver
Oct 23, 2015·PLoS Neglected Tropical Diseases·Jesse H ErasmusScott C Weaver
Feb 5, 2014·The American Journal of Tropical Medicine and Hygiene·Sarunyou ChusriRichard G Jarman
Oct 2, 2014·Expert Review of Vaccines·Karl Ljungberg, Peter Liljeström
Jul 23, 2015·Viruses·Mareike K S van Duijl-RichterJolanda M Smit
Oct 11, 2017·The American Journal of Tropical Medicine and Hygiene·Gillian EastwoodScott C Weaver
May 18, 2018·Nature·Rong ZhangMichael S Diamond
Dec 15, 2017·Clinical Microbiology Reviews·Ann M Powers
Aug 1, 2018·PLoS Neglected Tropical Diseases·Jing JinGraham Simmons
Dec 29, 2013·Journal of Virology·David HallengärdPeter Liljeström
Sep 12, 2014·Journal of Virology·David HallengärdPeter Liljeström
Feb 27, 2016·F1000Research·Lyle R Petersen, Ann M Powers
Feb 9, 2019·Human Vaccines & Immunotherapeutics·Arturo Reyes-Sandoval
Feb 17, 1998·Transactions of the Royal Society of Tropical Medicine and Hygiene·N JirakanjanakitN Bhamarapravati
Mar 2, 2017·The Journal of Clinical Investigation·Laurie A Silva, Terence S Dermody
Mar 31, 2019·Viruses·Jing Jin, Graham Simmons
Oct 30, 2019·Open Forum Infectious Diseases·Tamara L ClementsRandal J Schoepp
Jan 2, 2020·Scientific Reports·Emily M WebbAlbert J Auguste
Jan 18, 2019·PLoS Neglected Tropical Diseases·Giovanni Rezza, Scott C Weaver
Dec 7, 2016·The Journal of Infectious Diseases·Jesse H ErasmusScott C Weaver
Dec 7, 2016·The Journal of Infectious Diseases·Nicole N HaeseDaniel N Streblow
Oct 22, 2020·NPJ Vaccines·Christopher M WeissLark L Coffey
Aug 19, 2007·The Journal of General Virology·Ann M Powers, Christopher H Logue
Dec 5, 2020·Scientific Reports·Inga SzurgotPeter Liljeström
Feb 17, 2021·Virus Genes·Friederike I L HuckeJoachim J Bugert

Related Concepts

Metazoa
Animals, Suckling
Antibodies, Viral
Chikungunya virus
Cytopathogenic Effect, Viral
Macaca mulatta
Mouse, Inbred ICRC
Oligonucleotides
RNA, Viral
Togaviridae Infections

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Systemic Juvenile Idiopathic Arthritis

Systemic juvenile idiopathic arthritis is a rare rheumatic disease that affects children. Symptoms include joint pain, but also fevers and skin rashes. Here is the latest on this disease.

Chromatin Regulation and Circadian Clocks

The circadian clock plays an important role in regulating transcriptional dynamics through changes in chromatin folding and remodelling. Discover the latest research on Chromatin Regulation and Circadian Clocks here.

Central Pontine Myelinolysis

Central Pontine Myelinolysis is a neurologic disorder caused most frequently by rapid correction of hyponatremia and is characterized by demyelination that affects the central portion of the base of the pons. Here is the latest research on this disease.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.

Pontocerebellar Hypoplasia

Pontocerebellar hypoplasias are a group of neurodegenerative autosomal recessive disorders with prenatal onset, atrophy or hypoplasia of the cerebellum, hypoplasia of the ventral pons, microcephaly, variable neocortical atrophy and severe mental and motor impairments. Here is the latest research on pontocerebellar hypoplasia.

Cell Atlas Along the Gut-Brain Axis

Profiling cells along the gut-brain axis at the single cell level will provide unique information for each cell type, a three-dimensional map of how cell types work together to form tissues, and insights into how changes in the map underlie health and disease of the GI system and its crosstalk with the brain. Disocver the latest research on single cell analysis of the gut-brain axis here.

Chronic Traumatic Encephalopathy

Chronic Traumatic Encephalopathy (CTE) is a progressive degenerative disease that occurs in individuals that suffer repetitive brain trauma. Discover the latest research on traumatic encephalopathy here.