Development of an automated AmpliSeq™ library building workflow for biological stain samples on the Biomek® 3000.

BioTechniques
Maryam Sharafi FarzadClaus Børsting

Abstract

Here, we present the development of an automated AmpliSeq™ (ThermoFischer, MA, USA) workflow for library building using the Biomek® 3000 Laboratory Automation Workstation (Beckman Coulter Inc., CA, USA), in which the total volume of PCR reagents and reagents for library preparation are reduced by one-half. The automated AmpliSeq workflow was tested using 43 stain samples (blood, bone, muscle tissue, semen, swab, nail scrape and cigarette butts) collected from crime scenes. The sequencing data were evaluated for locus balance, heterozygous allele balance and noise. The performance of libraries built with the automated AmpliSeq workflow using one-half of the recommended reagent volumes were similar to the performance of libraries built with the recommended (full) volumes of the reagents.

References

Sep 13, 2011·Journal of Laboratory Automation·Michael StangegaardNiels Morling
Mar 26, 2013·Forensic Science International. Genetics·Claus BørstingNiels Morling
Nov 20, 2013·Investigative Genetics·Lutz Roewer
Feb 24, 2015·Forensic Science International. Genetics·Claus Børsting, Niels Morling
Jun 24, 2015·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·John M Butler
Oct 23, 2016·Forensic Science International. Genetics·Nicole M M NovroskiBruce Budowle
Nov 5, 2016·Electrophoresis·Anders BuchardNiels Morling
Sep 25, 2017·Forensic Science International. Genetics·Suzanne van der HeijdenNiels Morling
Feb 8, 2018·Forensic Science International. Genetics·Laurence DevesseDenise Syndercombe Court
May 26, 2018·International Journal of Legal Medicine·C HussingC Børsting

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Methods Mentioned

BETA
genotyping
PCR
electrophoresis
chip
chips

Software Mentioned

MPS
Excel
Torrent Suite Server
Biomek

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