Development of an automated sample preparation module for environmental monitoring of biowarfare agents

Analytical Chemistry
Benjamin J HindsonBilly W Colston

Abstract

An automated sample preparation module, based upon sequential injection analysis (SIA), has been developed for use within an autonomous pathogen detection system. The SIA system interfaced aerosol sampling with multiplexed microsphere immunoassay-flow cytometric detection. Metering and sequestering of microspheres using SIA was found to be reproducible and reliable, over 24-h periods of autonomous operation. Four inbuilt immunoassay controls showed excellent immunoassay and system stability over five days of unattended continuous operation. Titration curves for two biological warfare agents, Bacillus anthracis and Yersinia pestis, obtained using the automated SIA procedure were shown to be similar to those generated using a manual microtiter plate procedure.

References

Apr 16, 1999·Analytical Chemistry·J Ruzicka, L Scampavia
May 18, 1999·JAMA : the Journal of the American Medical Association·T V InglesbyK Tonat
Aug 8, 2002·Proteomics·Simon EkströmGyörgy Marko-Varga
Aug 28, 2002·The Analyst·Claire E LenehanSimon W Lewis

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Citations

Nov 12, 2005·Analytical and Bioanalytical Chemistry·Katie A EdwardsAntje J Baeumner
Oct 15, 2005·Clinical Microbiology Reviews·Daniel V LimMarianne F Kramer
Aug 11, 2011·PloS One·Christina M PrestonChristopher A Scholin
Nov 15, 2011·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Nobuaki SohToshihiko Imato
Aug 13, 2008·Analytical and Bioanalytical Chemistry·Geertruida A Posthuma-TrumpieAart van Amerongen
Apr 20, 2010·Journal of Microbiological Methods·Shilpakala Sainath RaoChintamani D Atreya
Mar 3, 2011·Journal of Forensic Sciences·Amanda BattagliaMargaret M Wallace
Oct 24, 2013·Journal of Forensic Sciences·Andrew J SchweighardtMargaret M Wallace
Mar 3, 2005·Biosensors & Bioelectronics·Benjamin J HindsonJohn M Dzenitis
Mar 22, 2014·Frontiers in Microbiology·Ekaterina OsmekhinaPeter Neubauer
Apr 12, 2015·Journal of Laboratory Automation·Christopher F Fronczek, Jeong-Yeol Yoon
Nov 5, 2004·Expert Review of Molecular Diagnostics·Nick M CirinoChristina Egan
Jan 30, 2008·The Analyst·Benjamin J HindsonJohn M Dzenitis
Jun 27, 2009·Immunopharmacology and Immunotoxicology·Jing WangRuifu Yang
Feb 28, 2007·The Analyst·Nicholas O FischerJeffrey B-H Tok
Nov 26, 2020·Toxins·Christopher Pöhlmann, Thomas Elßner

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