Abstract
An automated sample preparation module, based upon sequential injection analysis (SIA), has been developed for use within an autonomous pathogen detection system. The SIA system interfaced aerosol sampling with multiplexed microsphere immunoassay-flow cytometric detection. Metering and sequestering of microspheres using SIA was found to be reproducible and reliable, over 24-h periods of autonomous operation. Four inbuilt immunoassay controls showed excellent immunoassay and system stability over five days of unattended continuous operation. Titration curves for two biological warfare agents, Bacillus anthracis and Yersinia pestis, obtained using the automated SIA procedure were shown to be similar to those generated using a manual microtiter plate procedure.
References
Sep 1, 1997·Analytical Chemistry·B WillumsenJ Ruzicka
Mar 27, 1999·Analytical Chemistry·P S Hodder, J Ruzicka
Apr 16, 1999·Analytical Chemistry·J Ruzicka, L Scampavia
May 18, 1999·JAMA : the Journal of the American Medical Association·T V InglesbyK Tonat
Nov 27, 1999·Analytical Chemistry·I LähdesmäkiJ Ruzicka
Aug 12, 2000·Analytical Chemistry·P S HodderJ Ruzicka
Apr 18, 2001·Analytical Chemistry·K SatoT Kitamori
Jul 26, 2002·Analytical Chemistry·Gi Hun SeongRichard M Crooks
Aug 8, 2002·Proteomics·Simon EkströmGyörgy Marko-Varga
Aug 8, 2002·Proteomics·Jonas BergkvistThomas Laurell
Aug 28, 2002·The Analyst·Claire E LenehanSimon W Lewis
Dec 1, 1999·Analytical Chemistry·O B EgorovJ W Grate
Citations
Nov 12, 2005·Analytical and Bioanalytical Chemistry·Katie A EdwardsAntje J Baeumner
Apr 23, 2013·Analytica Chimica Acta·Dan LiMilton L Lee
Oct 15, 2005·Clinical Microbiology Reviews·Daniel V LimMarianne F Kramer
Aug 11, 2011·PloS One·Christina M PrestonChristopher A Scholin
Nov 15, 2011·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Nobuaki SohToshihiko Imato
Aug 13, 2008·Analytical and Bioanalytical Chemistry·Geertruida A Posthuma-TrumpieAart van Amerongen
Apr 20, 2010·Journal of Microbiological Methods·Shilpakala Sainath RaoChintamani D Atreya
Mar 3, 2011·Journal of Forensic Sciences·Amanda BattagliaMargaret M Wallace
Oct 24, 2013·Journal of Forensic Sciences·Andrew J SchweighardtMargaret M Wallace
Mar 3, 2005·Biosensors & Bioelectronics·Benjamin J HindsonJohn M Dzenitis
Mar 22, 2014·Frontiers in Microbiology·Ekaterina OsmekhinaPeter Neubauer
Apr 12, 2015·Journal of Laboratory Automation·Christopher F Fronczek, Jeong-Yeol Yoon
Nov 5, 2004·Expert Review of Molecular Diagnostics·Nick M CirinoChristina Egan
Jul 3, 2010·The Analyst·John ReganJames Birch
Jan 30, 2008·The Analyst·Benjamin J HindsonJohn M Dzenitis
Jun 27, 2009·Immunopharmacology and Immunotoxicology·Jing WangRuifu Yang
Feb 28, 2007·The Analyst·Nicholas O FischerJeffrey B-H Tok
Nov 26, 2020·Toxins·Christopher Pöhlmann, Thomas Elßner
Jun 18, 2009·Analytical Chemistry·Richard M OzanichJay W Grate
Sep 4, 2008·Analytical Chemistry·John F ReganJohn M Dzenitis
Dec 30, 2004·Analytical Chemistry·Benjamin J HindsonJohn M Dzenitis
Aug 16, 2006·Analytical Chemistry·Mei YangJian-Hua Wang
Jun 27, 2007·Analytical Chemistry·Jeanne C StachowiakVictoria A VanderNoot