Development of an efficient and simple method for conjugation of laccase to immunoglobulin and its characterization by enzyme immunoassay

Journal of Immunoassay & Immunochemistry
Swarnadeep RayBiswadev Bishayi

Abstract

A new laccase-conjugated antibody is developed, containing laccase as an enzyme marker, obtained from culture supernatant of the basidiomycetous fungi Pleurotus ostreatus. The efficacy of the laccase-conjugated antibody was demonstrated in indirect and direct enzyme immunoassay after using periodate and glutaraldehyde conjugation methods. The assay based on laccase-conjugated antibody is potent to detect antigens when compared with peroxidase conjugated antibody.

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Citations

Nov 13, 2013·Applied Biochemistry and Biotechnology·Gali Nirmal Kumar, Kotteazeth Srikumar

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